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DOI: 10.1055/s-0030-1250093
© Georg Thieme Verlag KG Stuttgart · New York
Manzamine A as a Novel Inhibitor of Herpes Simplex Virus Type-1 Replication in Cultured Corneal Cells
Publication History
received February 24, 2010
revised June 4, 2010
accepted June 7, 2010
Publication Date:
19 July 2010 (online)

Abstract
This study investigated the putative inhibitory effect of manzamine A on HSV-1 infection. Our results indicated that manzamine A effectively inhibited viral replication and infection in the cell line SIRC, a corneal cell line, at 1 µM. The existing anti-HSV-1 drug acyclovir was analyzed and showed a comparable activity at 50 µM. Plaque assays demonstrated that manzamine A reduced the release of infectious virus by 1011-fold. RTPCR assays indicated that HSV-1 virion host shutoff (vhs) activity and ICP0 transcription were decreased by manzamine A treatment. These results bode well for the development of manzamines as potential leads to reduce viral infection in corneal cells and to prevent HSV-1-induced eye infections such as keratitis.
Key words
manzamine A - herpes simplex virus - corneal cells - acyclovir
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Dr. Shao-chung Hsia
Department of Basic Pharmaceutical Sciences
University of Louisiana at Monroe College of Pharmacy
1800 Bienville Drive
Monroe, LA 71201
USA
Phone: +13 1 83 42 17 47
Fax: +13 1 83 42 17 37
Email: hsia@ulm.edu
Prof. Dr. Khalid El Sayed
Department of Basic Pharmaceutical Sciences
University of Louisiana at Monroe College of Pharmacy
1800 Bienville Drive
Monroe, LA 71201
USA
Phone: +13 1 83 42 17 25
Fax: +13 1 83 42 17 37
Email: elsayed@ulm.edu