Abstract
The 95 % ethanol extract of Astragalus has been demonstrated to have potent activity as an immunological adjuvant when administered with vaccines of various types. We endeavor here to identify the components of this extract that are responsible for this adjuvant activity. Mice were immunized with KLH conjugated to cancer carbohydrate antigens globo H and GD3 and cancer peptide antigen MUC1 combined with different Astragalus fractions or with commercially available Astragalus saponins and flavonoids. The antibody responses against cancer antigens and KLH were quantitated in ELISA assays, and toxicity was calculated by weight loss. Astragalosides II and IV were the most active components, but the toxicity of these two differed dramatically. Astragaloside II was the most toxic Astragalus component with 5–10 % weight loss at a dose of 500 µg while astragaloside IV showed no weight loss at all at this dose, suggesting that astragaloside IV might be utilized as an immunological adjuvant in future studies. Several flavonoids also had significant adjuvant activity. However, when the activities of these known immunologically active components of Astragalus (and of endotoxin) are calculated based on the extent of their presence in the 95 % ethanol extract, they provide only a small proportion of the immunological activity. This raises the possibility that additional uniquely active components of Astragalus may contribute to adjuvant activity, or that the adjuvant activity of Astragalus is greater than the activity of the sum of its parts.
Key words
Astragalus membranaceus
- Leguminosae - botanicals - conjugate vaccine - cancer vaccine - astragalosides - saponin
References
Astragalus membranaceus . Monograph.
Altern Med Rev.
2003;
8
72-77
Astragalus spp.) by ion trap LC-MS and nuclear ribosomal DNA sequence analyses.
J Agric Food Chem.
;
, , in press
Astragalus membranaceus in vivo .
J Clin Lab Immunol.
1988;
25
125-129
Panax notoginseng .
Vaccine.
2005;
23
5533-5542
Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice.
Vaccine.
2005;
23
5196-5203
Quillaja saponaria: synthesis of the potent clinical immunoadjuvant QS-21Aapi.
J Am Chem Soc.
2006;
128
11906-11915
livingsp@mskcc.org
