Abstract
Scrophularia ningpoensis Hemsl. is an important Chinese medicinal herb with a domestication history of more than one thousand years. Although a number of studies have focused on either chemical or genetic variation, none have dealt with their association to discuss the formation of chemical diversity. We applied HPLC fingerprinting with identification of four predominant bioactive compounds using LC‐ESI‐MS to assess chemical variation among 6 cultivated and 5 wild populations of S. ningpoensis . Significant chemical differences were revealed between wild and cultivated populations in terms of chromatographic profiles, principal component analysis (PCA) plots, and bioactive compounds contents. Compared to cultivated populations, the chemical profiles varied considerably among wild populations, of which some were remarkably similar to cultivated populations. Inter simple sequence repeats (ISSR) fingerprinting indicated a genetic differentiation pattern parallel to chemical variation. Evidence strongly supported the association between chemical and genetic variation of S. ningpoensis . Based on both sets of data, suggestions are proposed for the conservation of genetic diversity, crop improvement, and good agricultural practice. The present results will also facilitate our theoretical understanding of the selective and adaptive evolutionary processes of medicinal plant species impacted by domestication and a changing environment.
Key words
Scrophularia ningpoensis
- Scrophulariaceae - Radix Scrophulariae - chemical diversity - genetic diversity - ISSR fingerprinting - HPLC fingerprinting
References
Scrophularia scorodonia: in vitro anti-inflammatory activity.
Life Sci.
2004;
74
2515-2526
Scrophularia .
Pharm Biol.
2002;
40
45-59
Scrophularia frutescens L.
Farmaco.
1996;
51
443-446
Scrophulia ningpoensis in the Salmonella typhimurium TA1535/pSK1002 umu Test.
J Agric Food Chem.
1998;
46
904-910
Scrophularia ningpoensis Hemsl.
Pharm Biotechnol.
2007;
14
318-323
Scrophularian ningpoensis Hemsl. [dissertation]. Yangling; Northwest Agricultural Forestry University 2008
Scrophularia ningpoensis cultivars by SRAP.
China J Chin Mater Med.
2009;
34
138-142
DNA protocols for plants – CTAB total DNA isolation. Hewitt GM, Johnston A Molecular techniques in taxonomy. Heidelberg; Springer-Verlag 1991: 283-293
http://www.ualberta.ca/~fyeh Accessed October 3, 2009
http://www.eeb.uconn.edu Accessed October 3, 2009
Anemopsis californica .
Phytochemistry.
2008;
69
919-927
Anemopsis californica .
Hortscience.
2007;
42
1578-1583
Pinus ponderosa : a common garden study.
Ann Bot.
2009;
103
447-457
Vitex rotundifolia populations from different locations in China: its implication for quality control of medicinal plants.
Biomed Chromatogr.
2007;
21
967-975
Animal population genetics and disturbance: the effects of local extinctions and recolonizations on heterozygosity and fitness. Pickett STA, White PS The ecology of natural disturbance and path dynamics. London, MA; Academic Press 1985: 265-285
Isozymic evidence and the evolution of crop plants. Soltis DE, Soltis PS Isozymes in plant biology. Portland; Dioscorides Press 1989: 165-191
1 These two authors contributed to this work equally.
ypzhao@zju.edu.cn
cxfu@zju.edu.cn
