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Planta Med 2011; 77(9): 929-933
DOI: 10.1055/s-0030-1250672
Natural Product Chemistry
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Cytotoxic Steroidal Saponins from Agave sisalana

Pi-Yu Chen1 , Chin-Hui Chen1 , Ching-Chuan Kuo2 , Tzong-Huei Lee3 , Yueh-Hsiung Kuo4 , 5 , Ching-Kuo Lee1 , 3
  • 1School of Pharmacy, Taipei Medical University, Taipei, Taiwan
  • 2National Institute of Cancer Research, National Health Research Institutes, Tainan, Taiwan
  • 3Graduate Institute of Pharmacognosy Science, Taipei Medical University, Taipei, Taiwan
  • 4Tsutzki Institute for Traditional Medicine, China Medical University, Taichung, Taiwan
  • 5Agricultural Biotechnological Research Center, Academia Sinica, Taipei, Taiwan
Further Information

Publication History

received June 6, 2010 revised Nov. 15, 2010

accepted Dec. 7, 2010

Publication Date:
17 January 2011 (online)

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Abstract

Two new steroidal saponins, 8 and 10, along with 7 known steroidal sapogenins and saponins (1–7) and a furostanol saponin (9) were isolated from Agave sisalana Perrine ex Engelm. The structures of these two new compounds were identified and characterized by 1D and 2D NMR spectroscopy and mass spectrometry. In addition, acid hydrolysis and GC‐FID were used to confirm the sugar moieties of 8 and 10. The cytotoxic effects of 1–10 on MCF-7, NCI-H460, and SF-268 cancer cells were evaluated, and among them, compound 10 proved to be the most cytotoxic with IC50 values of 1.2, 3.8, and 1.5 µM, respectively.

Key words

Agavaceae - Agave sisalana - steroidal saponin - MCF‐7 cells - NCI‐H460 cells - SF‐268 cells

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Prof. Ching-Kuo Lee

School of Pharmacy, Taipei Medical University

No. 250 Wu-Xin Street

Taipei 110

Taiwan

Phone: +88 62 27 36 16 61 ext. 61 50

Fax: +88 62 23 77 22 65

Email: cklee@tmu.edu.tw

Prof. Yueh-Hsiung Kuo

Tsutzki Institute for Traditional Medicine
China Medical University

No. 91 Hsueh-Shih Road

Taichung 404

Taiwan

Phone: +88 64 22 05 33 66 ext. 57 01

Fax: +88 64 22 07 16 93

Email: yhkuo@ntu.edu.tw

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