The Plasminogen Activator Inhibitor-1 Gene Polymorphism in Determining the Risk of Pediatric Ischemic Stroke − Case Control and Family-Based Study

A. Balcerzyk; I. Żak; E. Emich-Widera; I. Kopyta; T. Iwanicki; E. Pilarska; K. Pienczk-Ręcławowicz; M. Kaciński; J. Wendorff; K. Połatyńska

doi:10.1055/s-0031-1279785

Neuropediatrics, Table of Contents

Neuropediatrics 2011; 42(02): 67-70
DOI: 10.1055/s-0031-1279785

Original Article

Georg Thieme Verlag Stuttgart · Newyork

The Plasminogen Activator Inhibitor-1 Gene Polymorphism in Determining the Risk of Pediatric Ischemic Stroke − Case Control and Family-Based Study

A. Balcerzyk

¹Department of Biochemistry and Medical Genetics, School of Health Care, Medical University of Silesia, Katowice, Poland

,

I. Żak

¹Department of Biochemistry and Medical Genetics, School of Health Care, Medical University of Silesia, Katowice, Poland

,

E. Emich-Widera

²Department of Neuropediatrics, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland

,

I. Kopyta

²Department of Neuropediatrics, School of Medicine in Katowice, Medical University of Silesia, Katowice, Poland

,

T. Iwanicki

¹Department of Biochemistry and Medical Genetics, School of Health Care, Medical University of Silesia, Katowice, Poland

,

E. Pilarska

³Department of Developmental Neurology, Medical University of Gdansk, Gdansk, Poland

,

K. Pienczk-Ręcławowicz

³Department of Developmental Neurology, Medical University of Gdansk, Gdansk, Poland

,

M. Kaciński

⁴Department of Pediatric and Adolescent Neurology, Jagiellonian University Medical College, Kraków, Poland

,

J. Wendorff

⁵Department of Neurology, Polish Mother’s Memorial Hospital − Research Institute, Łódź, Poland

,

K. Połatyńska

⁵Department of Neurology, Polish Mother’s Memorial Hospital − Research Institute, Łódź, Poland

› Author Affiliations

Abstract

Pediatric ischemic stroke, though relatively rare, remains an important medical problem since 20−40% of patients have recurrent strokes and 50−85% of them suffer from long-term neurological deficits. Approximately 20−50% of the affected children have prothrombotic disorders, therefore upon looking for possible genetic causes of the disease we focused on the plasminogen activator inhibitor (PAI-1) − the major inhibitor of fibrinolysis. The aim of the present study was to investigate a possible association between the -675_-674insG PAI-1 gene polymorphism and pediatric ischemic stroke. The study population consisted of 343 individuals: 70 children with ischemic stroke, 140 their biological parents and 133 control children. The PAI-1 gene polymorphism was genotyped using the restriction fragment length polymorphism and was visualized by AgNO₃ staining. The transmission/disequilibrium test showed exactly the same transmission of alleles from parents to the affected children (37:37). The case-control model also did not reveal any statistical significance in alleles and genotypes distribution between patients and control children. The obtained results suggest that the 4 G/5 G polymorphism of the PAI-I gene is not a risk factor of ischemic stroke in Polish children.

Key words

ischemic stroke - children - gene polymorphism - plasminogen activator inhibitor-1

Full Text

References

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