Planta Med 2012; 78(1): 79-81
DOI: 10.1055/s-0031-1280256
Natural Product Chemistry
Letters
© Georg Thieme Verlag KG Stuttgart · New York

Bioenhancing and Antimycobacterial Agents from Ammannia multiflora

Harish C. Upadhyay1 , Gaurav R. Dwivedi2 , Mahendra P. Darokar2 , Vinita Chaturvedi3 , Santosh K. Srivastava1
  • 1Analytical Chemistry Division, Central Institute of Medicinal and Aromatic Plants, P. O. CIMAP, Lucknow, India
  • 2Molecular and Bioprospection Division, Central Institute of Medicinal and Aromatic Plants, P. O. CIMAP, Lucknow, India
  • 3Drug Target Discovery and Development Division, Central Drug Research Institute, Lucknow, India
Further Information

Publication History

received May 9, 2011 revised August 26, 2011

accepted August 30, 2011

Publication Date:
27 September 2011 (online)

Abstract

The methanolic extract of Ammannia multiflora (Lythraceae) showed significant bioenhancing activity with the antibiotic nalidixic acid. Bioassay-guided fractionation of MeOH extract resulted in the isolation of a novel compound, 2,5-bis-(3,3′-hydroxyaryl)tetrahydrofuran, named as ammaniol (5), along with 9 other known compounds (1–4, 6–10). Furthermore, compound 4-hydroxy-α-tetralone (1) was converted into five semisynthetic acyl derivatives, 1A1E, which were evaluated along with compounds 1, 5, 6, 9, and 10 for their bioenhancing activity in combination with nalidixic acid against the two strains, CA8000 and DH5α, of Escherichia coli. The results showed that the methanolic extract of A. multiflora and compounds 1 and 9 possessed significant bioenhancing activity and reduced the dose of nalidixic acid fourfold while compounds 5, 6, 10 and semisynthetic derivatives 1A1E reduced the dose of nalidixic acid twofold. Compound 5 was also tested for antimycobacterial activity against Mycobacterium H37Rv and was found to show moderate activity (MIC 25 µg/mL) against this pathogen.

Supporting Information

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Santosh K. Srivastava

Analytical Chemistry Department
Central Institute of Medicinal and Aromatic Plants

P. O. CIMAP

Lucknow 226015

India

Phone: +91 52 22 35 96 23

Fax: +91 52 22 34 26 66

Email: santoshkumar_1955@yahoo.com