Esthesioneuroblastoma (ENB) is an uncommon malignant tumor derived from the specialized
neuroepithelium of the upper nasal cavity. Since its first description in 1924, almost
1000 cases of this tumor have been reported, mostly as single case reports.[1] ENB accounts for ~5% of intranasal cancers and shows a variety of symptoms and a
heterogeneous histopathology.[1]
[2] No data are presently available about either causal factors or factors associated
with a poor prognosis. The largest case series and meta-analyses suggest the combination
of surgery and radiotherapy as the gold standard treatment for ENB.[1]
[2]
[3]
[4]
[5]
It has been reported that the incidence of malignancies is increased among individuals
who are infected with human immunodeficiency virus (HIV). In recent years, while a
significant decrease in acquired immunodeficiency syndrome (AIDS) defining cancers
such as Kaposi sarcoma has been reported, patients infected with HIV remain at increased
risk for developing non-AIDS-related solid and hematological malignancies, an increasingly
prominent cause of death in the later stages of AIDS.[6]
[7]
[8]
[9]
The oncogenic role of Hepatitis C virus (HCV) infection in hepatocellular carcinoma
has been definitely established.[10] In addition, a possible role of this virus in the pathogenesis of extrahepatic neoplastic
disorders (B-cell malignancies) has been also suggested.[11]
We describe here, to the best of our knowledge, the first case of ENB in a young adult
man who was HIV-1 and HCV coinfected.
CASE REPORT
The patient was a 36-year-old man with a history of previous intravenous drug abuse;
he had been HIV-1 seropositive for 14 years and had never experienced opportunistic
infections. The CD4 cell count had always been higher than 350/mm3. The antiretroviral therapy was started 12 years after the diagnosis of HIV-1 infection,
and HIV-1 viral load was consistently undetectable during the past 5 years. Our patient
was also affected by chronic HCV infection in follow-up since 1992 and characterized
by a relatively low HCV viral load (~750.000 UI/mL), genotype 3b, and low-moderate
elevation of liver function tests. In April 2004, the patient presented an acute onset
of right maxillary swelling, for which he was admitted to our Infectious Diseases
ward. Magnetic resonance imaging (MRI) revealed a tumor filling almost completely
the upper right nasal cavity and ethmoid, reaching the cribriform plate but without
intracranial involvement; there was initial involvement of the medial wall of the
right orbit, with displacement of the retrobulbar orbital fat but without evidence
of extension into the orbit (Fig. [1]). No regional and systemic metastases were found at that moment by computed tomography
(CT) evaluation. Histological examination of the mass revealed diffuse sheets of neoplastic
cells with lobular pattern and prominent background of capillaries, but little intervening
stroma. The neoplastic cells had high nucleus-to-cytoplasm ratio and often showed
rosettes formation. Scattered and rare mitoses were observed, there was no necrotic
area present. Immunohistochemical staining showed that the tumor was positive for
synaptophysin, neuron-specific enolase, chromogranin, and CD56, and negative for keratin,
lymphoid markers, and epithelial membrane antigen; some S-100 protein-positive sustentacular
cells were also seen. On the basis of these features, a diagnosis of ENB was made.
According to Hyams' histopathological grading,[1] the tumor was classified as grade II. Based on staging classification systems of
Kadish and Dulguerov,[1] tumor was classified as stage B and T2N0M0, respectively. Because of stage and comorbidities,
patient underwent a minimally invasive endoscopic resection (MIER) of the tumor by
means of antrostomy, ethmoidectomy, and sphenoidectomy.
Figure 1 Preoperative MRI: (A) Axial T1-weigthed images without contrast; (B) Axial T2-weigthed
images; (C) Coronal T2-weighted images; (D) Axial fat-saturated T1-weighted images
after contrast; (E) Sagittal fat-saturated T1-weighted images after contrast; (F)
Coronal fat-saturated T1-weighted images after contrast. The tumor fills almost completely
the upper right nasal cavity and ethmoid, reaching the cribriform plate but without
intracranial involvement; there was initial involvement of the medial wall of the
right orbit, with displacement of the retrobulbar orbital fat but without evidence
of extension into the orbit. The tumor showed relatively low signal on T2-weighted
images and moderate enhancement due to its high cellularity. Combination of T2-weighted
and postcontrast fat-saturated T1-weighed images allowed good differentiation between
the tumor itself and the associated reactive/obstructive findings in the right maxillary,
frontal, sphenoid sinuses.
Subsequently, the patient received 55 Gy of external beam radiotherapy on the resected
area from the nasal cavity to the adjacent meninges over a 6-week period and chemotherapy
with cisplatinum (three cycles of 40 mg/m2 per day for 5 days every 3 weeks). A cranial MRI performed 6 months later showed
no residual tumor; this finding was confirmed by two different histological examinations
performed on local biopsies.
In June 2005, right upper cervical lymphadenopathy was noted; MRI showed the presence
of an extensive lymph node metastasis infiltrating the carotid sheath. The cytologic
diagnosis performed by fine needle aspiration (FNA) of the mass confirmed ENB relapse;
MRI did not show signs of local recurrence. The patient underwent a new treatment
of external beam radiotherapy on the metastatic area (total dose 50 Gy over a 6-week
period). The subsequent MRI showed partial reduction of the mass which had no cleavage
planes with the adjacent muscles. Simultaneously, the patient developed lumbar pain.
MRI and CT showed regional and multiple distant metastases localized at bones (thoracic,
lumbar, and sacral vertebrae, ileum, sacrum, and thighbones), at liver, and at abdominal
and pelvic lymph nodes. The cytologic examination performed by FNA of one of the hepatic
nodules confirmed the diagnosis of ENB metastases; the immunohistochemistry showed
the same pattern of the primitive tumor. Due to the underlying persistent leukopenia
(white blood cell count <2000/mm3), thus making the use of polychemotherapy regimens contraindicated, the patient was
treated with four cycles of cisplatinum (60 mg/m2 per week), but a new MRI and CT staging showed a dramatic progression of the neoplasm.
The patient died in July 2006.
DISCUSSION
With the advent of highly active antiretroviral therapy, the morbidity and mortality
associated with HIV infection dramatically reduced, resulting in a large number of
older individuals living with HIV infection and in increasing incidence of non-AIDS-related
malignancies.[6] In large database of AIDS-associated cancer, the overall rates of several non-AIDS-related
neoplasms (including Hodgkin lymphoma, invasive anal carcinoma, multiple myeloma,
leukemia, lung cancer, as well as other solid malignancies), were found to be higher
in patients infected with HIV than in general population.[8] However, unlike AIDS-defining malignancies, no association has been established
between many non-AIDS-defining cancers and specific risk factors. In addition, some
studies reported the absence of correlation between CD4 cell count and development
of solid non-AIDS-defining cancers.[7]
[9]
Neither data nor risk factors about clear etiological agents for ENB in human beings
are presently available. However, type C retrovirus particles have been demonstrated
in the tumor rosettes of spontaneous ENB from domestic cats as well as in transgenic
mice developing ENB.[1] Although the role of retroviral sequences in human ENB remains unclear and no association
between HIV-1 and this neoplasm has been established, it can be hypothesized that
HIV-1 retroviral activity may play a role as a cofactor in the development of ENB.
Furthermore, it is noteworthy that our patient was coinfected with HCV, a well-recognized
oncogenic factor.
Although ENB can be locally aggressive and metastasize by lymphatic and hematogenous,
distant metastases and very poor prognosis are not common (less then 10% of patients);
the largest studies reported a 5-year survival around 45%.[1]
[2]
[3]
[4]
[5]
Interestingly, in two recent studies of 30 and 18 cases of ENB, respectively, the
frequency of recurrence was ~30%, no distant metastases were observed, and the overall
10-year disease-specific survival was 81% and 80%, respectively.[3]
[4] In both of these studies, the standard therapeutic approach included surgery, mostly
combined with preoperative or postoperative radiotherapy; the chemotherapy was used
in patients with advanced initial stages or with recurrent disease. Similarly, the
recent and largest case series published by Jethanamest and colleagues[5] including 311 patients with ENB, reported the overall and disease-specific survival
rates at 10 years to be 49% and 68.3%, respectively, and an overall mean survival
time of 149.5 months for patients with Kadish B stage disease; no patients received
treatment with chemotherapy in this study. In contrast with these data, in our case,
although the combination of surgery and postoperative radiotherapy and chemotherapy
constituted the initial approach, and despite the early stage of the disease, the
patient developed regional and then distant metastases after ~12 months, with a rapid
poor outcome.
Concerning the surgical approach, although the craniofacial resection (CFR) is still
considered the gold standard for surgical resection in treatment of ENB, postoperative
morbidity (~35%) and mortality (2 to 5%) related to this procedure remain relatively
high.[4] Since the MIER was introduced by Stammberger et al in 1999 as an alternative to
CFR in early stages (A, B, T1, and T2 tumors), an increasing number of case series
have reported excellent survival and few complications with MIER of EBN in selected
patients.[4] It is not yet clear if MIER approach enables an adequate margin of resection and,
if not, whether this matters, particularly if adjuvant radiotherapy is given.[2] In addition, although there is a lack of consensus about the prognostic value of
histologic features of this tumor, it has been reported that Hyams and coworkers'
grading could represent an important factor affecting prognosis.[12] Noteworthy, poorly differentiated tumors might have already metastasized and it
could be considered reasonable to minimize surgical morbidity and mortality using
MIER, rather than CFR.[13] Adjuvant radiation therapy for ENB has been reported to improve local tumor control,
particularly for high-grade and high-stage tumors[12]; in addition, it has been suggested that cisplatin-based chemotherapy is active
against ENB, even in advanced, high-grade stages.[1]
[2] Despite the early grade and stage of the disease, and taking into account our patient's
comorbidities, adjuvant radiotherapy and monochemotherapy with cisplatin were considered
reasonably indicated as consolidation treatment after surgery to optimize the patient's
survival.
Although this is the only one case of ENB in an HIV-1 infected patient described until
now, the rapid and aggressive progression of this cancer in our patient suggests that
in HIV-1 infected patients the possibility of a more intrinsic aggressiveness of the
tumor, as documented by rapid development of distant metastases and poor prognosis
also in presence of a well-established and prolonged immune reconstitution, should
be taken into account. Several studies have shown that some malignancies tend to present
with a more aggressive clinical course in HIV-positive patients, when compared with
the same neoplastic processes in HIV-negative patients, and the absence of correlation
between CD4 cell count and development of solid non-AIDS-defining cancers has been
reported.[7]
[8] In addition, the role of chemotherapy, radiation therapy, and surgery for treatment
of non-AIDS-related malignancies in HIV-1 infected patients remains unclear.[6]
Until larger numbers of cases ideally controlled for stage and differentiation and
with longer follow-up will confirm the opportunity to treat endoscopically in conjunction
with radiotherapy early stages (A, B, T1, T2) of EBN, our case seems to suggest that
in HIV-1 infected patients a more aggressive therapeutic approach to ENB at the onset,
with en-bloc CFR, and adjuvant radiotherapy (including the possible use of concomitant
chemotherapy, usually reserved to patients with recurrent or metastatic disease),
should be considered. Furthermore, it could be reasonable to consider the use a more
frequent and intensive clinical and radiological follow-up and the use of combination
chemotherapy regimens.
