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Exp Clin Endocrinol Diabetes 2012; 120(02): 101-109
DOI: 10.1055/s-0031-1284432
Article
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Generation of T Regulatory Cells in Children with Newly Diagnosed Type 1 Diabetes Mellitus

W. Łuczyński
1   Department of Pediatrics, Endocrinology, Diabetology with Cardiology Division, Poland
,
N. Wawrusiewicz-Kurylonek
2   Department of Endocrinology, Diabetology and Internal Medicine, Poland
,
A. Szypowska
3   Department of Pediatrics, Medical University of Warsaw. Poland
,
E. Iłendo
4   Department of Pediatric Laboratory Diagnostics, Poland
,
A. Bossowski
1   Department of Pediatrics, Endocrinology, Diabetology with Cardiology Division, Poland
,
A. Krętowski
2   Department of Endocrinology, Diabetology and Internal Medicine, Poland
,
A. Stasiak-Barmuta
5   Department of Clinical Immunology Medical University of Bialystok, Poland
› Author Affiliations
Further Information

Publication History

received 13 April 2011
first decision 13 April 2011

accepted 21 July 2011

Publication Date:
15 September 2011 (online)

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Abstract

There is increasing evidence that T-regulatory (Treg) cells could be used to prevent or cure autoimmune diseases including type 1 diabetes mellitus (T1DM). The aim of the present study was to verify the hypothesis that functional Treg cells can be generated from conventional T-cells separated from a small amount of peripheral blood of children with newly diagnosed T1DM (N=25).

Methods:

CD4+CD25- cells were cultured with Treg expander (CD3/CD28) and IL-2 for generating de novo Treg cells. The assessment of the expression of selected genes and proteins critical to Treg function and the proliferation assays were performed with the use of real-time RT-PCR and flow cytometry.

Results:

After a 4-week stimulation with Treg expander and IL-2, the percentage of T-regulatory cells was significantly higher compared to the cells treated with medium alone (with no difference between diabetic and control children). However, we found some disturbances in the gene expression at mRNA level for molecules crucial for T-reg function. The induced Tregs from diabetic and control children were fully functional as assessed in proliferation assays.

In summary:

despite some disturbances at mRNA level in the critical gene expression, the suppressive properties of induced Treg cells from diabetic and control children were effective.

Key words

T regulatory cell - diabetes - children - immunotherapy - mRNA
 

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