Generation of T Regulatory Cells in Children with Newly Diagnosed Type 1 Diabetes Mellitus

W. Łuczyński; N. Wawrusiewicz-Kurylonek; A. Szypowska; E. Iłendo; A. Bossowski; A. Krętowski; A. Stasiak-Barmuta

doi:10.1055/s-0031-1284432

Experimental and Clinical Endocrinology & Diabetes, Table of Contents

Exp Clin Endocrinol Diabetes 2012; 120(02): 101-109
DOI: 10.1055/s-0031-1284432

Article

Generation of T Regulatory Cells in Children with Newly Diagnosed Type 1 Diabetes Mellitus

W. Łuczyński

¹Department of Pediatrics, Endocrinology, Diabetology with Cardiology Division, Poland

,

N. Wawrusiewicz-Kurylonek

²Department of Endocrinology, Diabetology and Internal Medicine, Poland

,

A. Szypowska

³Department of Pediatrics, Medical University of Warsaw. Poland

,

E. Iłendo

⁴Department of Pediatric Laboratory Diagnostics, Poland

,

A. Bossowski

¹Department of Pediatrics, Endocrinology, Diabetology with Cardiology Division, Poland

,

A. Krętowski

²Department of Endocrinology, Diabetology and Internal Medicine, Poland

,

A. Stasiak-Barmuta

⁵Department of Clinical Immunology Medical University of Bialystok, Poland

› Author Affiliations

Abstract

There is increasing evidence that T-regulatory (Treg) cells could be used to prevent or cure autoimmune diseases including type 1 diabetes mellitus (T1DM). The aim of the present study was to verify the hypothesis that functional Treg cells can be generated from conventional T-cells separated from a small amount of peripheral blood of children with newly diagnosed T1DM (N=25).

Methods:

CD4⁺CD25^- cells were cultured with Treg expander (CD3/CD28) and IL-2 for generating de novo Treg cells. The assessment of the expression of selected genes and proteins critical to Treg function and the proliferation assays were performed with the use of real-time RT-PCR and flow cytometry.

Results:

After a 4-week stimulation with Treg expander and IL-2, the percentage of T-regulatory cells was significantly higher compared to the cells treated with medium alone (with no difference between diabetic and control children). However, we found some disturbances in the gene expression at mRNA level for molecules crucial for T-reg function. The induced Tregs from diabetic and control children were fully functional as assessed in proliferation assays.

In summary:

despite some disturbances at mRNA level in the critical gene expression, the suppressive properties of induced Treg cells from diabetic and control children were effective.

Key words

T regulatory cell - diabetes - children - immunotherapy - mRNA

Full Text

References

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