Aldosterone does not Modify Gene Expression in Human Endothelial Cells

A. Verhovez; T. A. Williams; F. Morello; S. Monticone; M. F. Brizzi; P. Dentelli; F. Fallo; B. Fabris; F. Amenta; C. Gomez-Sanchez; F. Veglio; P. Mulatero

doi:10.1055/s-0031-1291272

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2012; 44(03): 234-238
DOI: 10.1055/s-0031-1291272

Original Basic

Aldosterone does not Modify Gene Expression in Human Endothelial Cells

A. Verhovez

¹Division of Internal Medicine and Hypertension, Department of Medicine and Experimental Oncology, University of Torino, Torino, Italy

,

T. A. Williams

¹Division of Internal Medicine and Hypertension, Department of Medicine and Experimental Oncology, University of Torino, Torino, Italy

,

F. Morello

¹Division of Internal Medicine and Hypertension, Department of Medicine and Experimental Oncology, University of Torino, Torino, Italy

,

S. Monticone

¹Division of Internal Medicine and Hypertension, Department of Medicine and Experimental Oncology, University of Torino, Torino, Italy

,

M. F. Brizzi

²Department of Internal Medicine, University of Torino, Torino, Italy

,

P. Dentelli

²Department of Internal Medicine, University of Torino, Torino, Italy

,

F. Fallo

³Department of Medical and Surgical Sciences, University of Padova, Padova, Italy

,

B. Fabris

⁴Department of Medical, Technological and Translational Sciences, University of Trieste, Cattinara Hospital, Trieste, Italy

,

F. Amenta

⁵Section of Human Anatomy, School of Pharmacy, University of Camerino, Camerino (MC), Italy

,

C. Gomez-Sanchez

⁶Division of Endocrinology, G.V. (Sonny) Montgomery VA Medical Center and University of Mississippi Medical Center, Jackson, MS, USA

,

F. Veglio

¹Division of Internal Medicine and Hypertension, Department of Medicine and Experimental Oncology, University of Torino, Torino, Italy

,

P. Mulatero

¹Division of Internal Medicine and Hypertension, Department of Medicine and Experimental Oncology, University of Torino, Torino, Italy

› Author Affiliations

Abstract

The toxic effects of aldosterone on the vasculature, and in particular on the endothelial layer, have been proposed as having an important role in the cardiovascular pathology observed in mineralocorticoid-excess states. In order to characterize the genomic molecular mechanisms driving the aldosterone-induced endothelial dysfunction, we performed an expression microarray on transcripts obtained from both human umbilical vein endothelial cells and human coronary artery endothelial cells stimulated with 10^− 7 M aldosterone for 18 h. The results were then subjected to qRT-PCR confirmation, also including a group of genes known to be involved in the control of the endothelial function or previously described as regulated by aldosterone. The state of activation of the mineralocorticoid receptor was investigated by means of a luciferase-reporter assay using a plasmid encoding a mineralocorticoid and glucocorticoid-sensitive promoter. Aldosterone did not determine any significant change in gene expression in either cell type both in the microarray and in the qRT-PCR analysis. The luciferase-reporter assay showed no activation of the mineralocorticoid receptor following aldosterone stimulation. The status of nonfunctionality of the mineralocorticoid receptor expressed in cultured human umbilical and coronary artery endothelial cells does not allow aldosterone to modify gene expression and provides evidence against either a beneficial or harmful genomic effect of aldosterone on healthy endothelial cells.

Key words

mineralocorticoid - endothelial dysfunction - genomic

Full Text

References

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