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DOI: 10.1055/s-0031-1298146
© Georg Thieme Verlag KG Stuttgart · New York
Reversal of Multidrug Resistance of KBV200 Cells by Triterpenoids Isolated from Poria cocos
Publication History
received Nov. 9, 2011
revised Dec. 5, 2011
accepted Dec. 9, 2011
Publication Date:
13 January 2012 (online)
![](https://www.thieme-connect.de/media/plantamedica/201205/lookinside/thumbnails/10.1055-s-0031-1298146-1.jpg)
Abstract
Multidrug resistance (MDR) of tumor cells is one of the major problems encountered during cancer chemotherapy. In this paper, we isolated eight triterpenoids from Poria cocos and evaluated their effects on reversing MDR of KBV200 cells. Eight triterpenoids increase significantly vincristine-induced cytotoxicity in drug-resistant KBV200 cells at the concentrations of 12.5 µg/mL and 25 µg/mL. Dehydrotumulosic acid showed the best reversal effect: it increased KBV200 apoptosis induced by vincristine and inhibited P-gp function through enhancing the accumulation and retention of fluorescent P-gp substrate rhodamine 123 in KBV200 cells but had no effect on P-gp expression.
Key words
Poria cocos - Polyporaceae - triterpenoids - MDR - KBV200 cells - P-gp
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Prof. Qinglin Zhang
Department of Experimental Hematology
Beijing Institute of Radiation Medicine
Taiping Road 27
Beijing 100850
People's Republic of China
Phone: +86 10 66 93 02 70
Fax: +86 10 66 93 02 70
Email: qinglz@yahoo.com