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Arzneimittelforschung 2012; 62(07): 340-344
DOI: 10.1055/s-0032-1312602
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

In vitro and in vivo Evaluation of Triptolide-loaded Pluronic P105 Polymeric Micelles

H. Li
1   Department of Chinese Traditional Medicine, Renji Hospital Jiaotong University School of Medicine, Shanghai, China
,
X.-S. Wen
1   Department of Chinese Traditional Medicine, Renji Hospital Jiaotong University School of Medicine, Shanghai, China
,
W. Di
1   Department of Chinese Traditional Medicine, Renji Hospital Jiaotong University School of Medicine, Shanghai, China
› Author Affiliations
Further Information

Publication History

received 03 March 2012

accepted 20 April 2012

Publication Date:
15 May 2012 (online)

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Abstract

Thin film method was applied successfully to prepare Triptolide (TP)-loaded micelles system. With a dynamic light scattering sizer and a transmission electron microscopy, it was shown that the TP-loaded micelles had a mean size of 84.3±6.4 nm with a spherical shape. The in vitro release profiles indicated that the release of TP from the micelles exhibited a sustained release behavior. A similar phenomenon was also observed in a pharmacokinetic study in rats, in which AUC of the micelles formulation were 4.7-fold higher than that of TP injection. The biodistribution study in rats showed that the TP-loaded micelles not only decreased drug uptake by liver, but also increased distribution of drug in ovary. The present work demonstrated the feasibility of controlled delivery of TP utilizing micelles system.

Key words

TP-loaded micelles - in vitro release - pharmacokinetic study - biodistribution study
 

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