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Planta Med 2012; 78(14): 1584-1590
DOI: 10.1055/s-0032-1315040
Natural Product Chemistry
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Triterpene Acids from Euscaphis japonica and Assessment of Their Cytotoxic and Anti-NO Activities

Li-Jie Zhang
1   National Research Institute of Chinese Medicine, Taipei, Taiwan
,
Jing-Jy Cheng
1   National Research Institute of Chinese Medicine, Taipei, Taiwan
2   Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan
,
Chia-Ching Liao
1   National Research Institute of Chinese Medicine, Taipei, Taiwan
,
Hui-Ling Cheng
1   National Research Institute of Chinese Medicine, Taipei, Taiwan
,
Hung-Tse Huang
1   National Research Institute of Chinese Medicine, Taipei, Taiwan
,
Li-Ming Yang Kuo
1   National Research Institute of Chinese Medicine, Taipei, Taiwan
,
Yao-Haur Kuo
1   National Research Institute of Chinese Medicine, Taipei, Taiwan
3   Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan
› Author Affiliations
Further Information

Publication History

received 18 February 2012
revised 11 June 2012

accepted 20 June 2012

Publication Date:
19 July 2012 (online)

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Abstract

Six new triterpenoids, euscaphic acids G–L (1–6), along with nine known triterpene acids, and two known lignans were isolated from the ethanolic extract of the twigs of Euscaphis japonica. This is the first report concerning 1α,3β-dihydroxy-12-oleanen-28-oic acid isolated from a natural source. The structures of the new compounds were established by spectroscopic analysis. The cytotoxic and anti-NO production activities for the isolates are also evaluated and discussed; compound 1, hederagenin (11), and arjunic acid (12) showed significant cytotoxicity against NCI-H460 cells, HT-29 cells, and CEM cells (IC50 = 1.64 ± 0.87, 2.11 ± 1.54, 1.73 ± 0.64 µM, respectively). Some of the isolated triterpenoids showed marginal inhibitions on NO production induced by LPS.

Key words

Euscaphis japonica - Staphyleaceae - euscaphic acids G–L - triterpenoids - cytotoxicity - anti-NO production

Supporting Information

 

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