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Rofo 2013; 185(1): 60-65
DOI: 10.1055/s-0032-1325406
Neuroradiologie
© Georg Thieme Verlag KG Stuttgart · New York

Impact of Protein Content on Proton Diffusibility in Intracranial Cysts

Einfluss des Proteingehalts auf Diffusionsphänomene in intrakraniellen Zysten
E. Goebell
1   Neuroradiology, University Medical Center Hamburg-Eppendorf
,
J. Fiehler
1   Neuroradiology, University Medical Center Hamburg-Eppendorf
,
T. Martens
2   Neurosurgery, University Medical Center Hamburg-Eppendorf
,
C. Hagel
3   Neuropathology, University Medical Center Hamburg-Eppendorf
,
N. D. Forkert
4   Institute for Computational Neuroscience, University Medical Center Hamburg-Eppendorf
,
A. Russjan
5   Neurology, University Medical Center Hamburg-Eppendorf
,
M. Rosenkranz
5   Neurology, University Medical Center Hamburg-Eppendorf
,
J.-H. Buhk
1   Neuroradiology, University Medical Center Hamburg-Eppendorf
,
M. Groth
1   Neuroradiology, University Medical Center Hamburg-Eppendorf
,
J. Sedlacik
1   Neuroradiology, University Medical Center Hamburg-Eppendorf
› Author Affiliations
Further Information

Publication History

17 February 2012

28 August 2012

Publication Date:
11 October 2012 (online)

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Abstract

Purpose: The physical background of diffusion phenomena in intracranial cysts is unclear in some cases. To evaluate a potential dependency of proton diffusion on the concentration of proteins in cystic lesions we investigated the correlation of diffusion weighted imaging (DWI) and magnetization transfer ratio imaging (MTR) in intracranial cystic pathologies in vivo and in vitro with protein solutions.

Materials and Methods: 21 patients (14 male/7 female) with intracranial cystic lesions underwent preoperative MRI (1.5 T) including MTR and DWI sequences. For comparison a series of samples with declining concentration of albumin was investigated in vitro with a 7 T animal scanner.

Results: In the patients examination mean ADC values were 1.93 × 10−3 mm2/sec and mean MTR values were 0.2. Mean ADC value of the albumin solutions was 0.22 × 10−3 mm2/sec and mean MTR was 0.12. ADC and MTR values showed a strong negative correlation in the patients (Spearman’s rank correlation rs = –0.80, p < 0.01) and a very strong negative correlation in the in vitro examinations (rs = –1.0, p < 0.01).

Conclusion: The strong negative correlation of ADC and MTR values suggest a strong influence of proteins on proton diffusion in intracranial cysts. The phenomena can be explained by macromelecules that bind nearby protons in their vicinity.

Zusammenfassung

Ziel: Die physikalische Grundlage von Diffusionsphänomenen intrakranieller Raumforderungen ist in vielen Fällen unbekannt. Um einen möglichen Einfluss des Proteingehalts in intrakraniellen Zysten auf die Protonendiffusion zu untersuchen, wurde die Korrelation von Diffusionsdaten (DWI) und Magnetisation Transfer Ratio Imaging (MTR) in intrakraniellen Zysten und in vitro untersucht.

Material und Methoden: 21 Patienten (m/w: 14/7) mit intrakraniellen zystischen Läsionen wurden präoperativ mit ADC- und MTR-Bildgebung kernspintomografisch (1,5 T) untersucht. Vergleichend wurde eine Verdünnungsreihe mit Albuminlösungen, in vitro, in einem 7 T MRT-Gerät untersucht.

Ergebnisse: Der durchschnittliche ADC-Wert in den zystischen Läsionen betrug 1,93 × 10−3 mm2/s und der durchschnittliche MTR-Wert 0,20. Der durchschnittliche ADC-Wert in den Albuminlösungen betrug 0,22 × 10−3 mm2/s und der durchschnittliche MTR-Wert 0,12. Es zeigte sich eine deutliche negative Korrelation der ADC- und MTR-Werte in den verschiedenen zystischen Läsionen (Spearman Rang Korrelation rs = –0,80, p < 0,01) und eine sehr starke, negative Korrelation in der Verdünnungsreihe der Proteinlösungen (rs = –1,0, p < 0,01).

Schlussfolgerung: Die deutliche negative Korrelation der ADC- und MTR-Werte belegt einen starken Einfluss des Proteingehalts auf die Protonendiffusibilität in zystischen intrakraniellen Läsionen. Das Phänomen kann durch die Bindung des Wassers an Makromoleküle erklärt werden.

Key words

CNS - MR-imaging - Abscess - Diffusion imaging
 

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