Downregulation of Endogenous Intermedin Augmented Myocardial Injury in Rats with Ischemia/Reperfusion

X. Teng; Y. Bian; Y. Cai; X. Duan; F. Yuan; J. Du; W. Wu; X. Wang; C. Tang; Y. Qi

doi:10.1055/s-0032-1327572

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 2013; 45(03): 206-212
DOI: 10.1055/s-0032-1327572

Original Basic

Downregulation of Endogenous Intermedin Augmented Myocardial Injury in Rats with Ischemia/Reperfusion

X. Teng^*

¹The Key Laboratory of Remodeling-related Cardiovascular Diseases, Capital Medical University, Ministry of Education, Beijing, China

²Department of Physiology, Hebei Medical University, Shijiazhuang, China

³Hebei Key Laboratory of Laboratory Animal Science, Shijiazhuang, Hebei, China

,

Y. Bian^*

⁴Department of Cardiology, the Second Hospital of Shanxi Medical University, Taiyuan, Shanxi, China

,

Y. Cai

¹The Key Laboratory of Remodeling-related Cardiovascular Diseases, Capital Medical University, Ministry of Education, Beijing, China

,

X. Duan

⁵Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

,

F. Yuan

²Department of Physiology, Hebei Medical University, Shijiazhuang, China

,

J. Du

¹The Key Laboratory of Remodeling-related Cardiovascular Diseases, Capital Medical University, Ministry of Education, Beijing, China

,

W. Wu

⁵Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

,

X. Wang

⁵Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

,

C. Tang

¹The Key Laboratory of Remodeling-related Cardiovascular Diseases, Capital Medical University, Ministry of Education, Beijing, China

⁵Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

,

Y. Qi

¹The Key Laboratory of Remodeling-related Cardiovascular Diseases, Capital Medical University, Ministry of Education, Beijing, China

⁵Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Beijing, China

› Institutsangaben

Abstract

Intermedin (IMD) plays an important regulatory role in cardiovascular function. We aimed to explore the protein expression of IMD and its receptors, calcitonin receptor-like receptor (CRLR) and receptor activity-modifying proteins (RAMPs), and the role of endogenous IMD in myocardial ischemia/reperfusion (I/R) injury in rats. The rat model of I/R was created by ligating cardiac left anterior descending artery. Western blot was used to determine protein expression of CRLR and RAMPs, and radioimmunoassay was used to detect IMD content. Compared with control, protein levels of CRLR and RAMPs in both ischemic and nonischemic region were upregulated at different stages of reperfusion. IMD protein content in nonischemic area myocardium also increased. However, IMD protein content in ischemic area downregulated at 3-, 6-, and 12-h reperfusion. In hypoxia/reoxygenation model of neonatal cardiomyocytes, IMD attenuated myocyte injury, and IMD receptor antagonist IMD_17–47 aggravated myocyte impairment by blocking endogenous IMD. In conclusion, the downregulation of IMD at early stage of reperfusion might augment myocardium injury.

Key words

calcitonin receptor-like receptor - receptor activity-modifying proteins - cardiomyocytes - hypoxia/reoxygenation injury

Volltext

Referenzen

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