Inhibition of Lipopolysaccharide-Induced iNOS and COX-2 Expression by
                    Indole Alkaloid, 3-(Hydroxymethyl)-6,7-Dihydroindolo[2,3-a]Quinolizin-(12H)-one,
                    via NF-κB Inactivation in RAW 264.7 Macrophages

Feng Zhao; Lei Chen; Menglin Zhang; Chenchen Bi; Lichun Li; Qingzhen Zhang; Cuicui Shi; Miao Li; Songsong Zhou; Linghua Kong

doi:10.1055/s-0032-1328550

Planta Medica, Table of Contents

Planta Med 2013; 79(09): 782-787
DOI: 10.1055/s-0032-1328550

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Inhibition of Lipopolysaccharide-Induced iNOS and COX-2 Expression by Indole Alkaloid, 3-(Hydroxymethyl)-6,7-Dihydroindolo[2,3-a]Quinolizin-(12H)-one, via NF-κB Inactivation in RAW 264.7 Macrophages

Feng Zhao

¹School of Pharmacy, Yantai University, Yantai, PR China

,

Lei Chen

¹School of Pharmacy, Yantai University, Yantai, PR China

,

Menglin Zhang

¹School of Pharmacy, Yantai University, Yantai, PR China

,

Chenchen Bi

¹School of Pharmacy, Yantai University, Yantai, PR China

,

Lichun Li

¹School of Pharmacy, Yantai University, Yantai, PR China

,

Qingzhen Zhang

¹School of Pharmacy, Yantai University, Yantai, PR China

,

Cuicui Shi

¹School of Pharmacy, Yantai University, Yantai, PR China

,

Miao Li

¹School of Pharmacy, Yantai University, Yantai, PR China

,

Songsong Zhou

¹School of Pharmacy, Yantai University, Yantai, PR China

,

Linghua Kong

¹School of Pharmacy, Yantai University, Yantai, PR China

› Author Affiliations

Abstract

3-(Hydroxymethyl)-6,7-dihydroindolo[2,3-a]quinolizin-(12H)-one is a bioactive indole alkaloid isolated from Nauclea officinalis, a plant species which is used as a traditional Chinese medicine. We investigated the anti-inflammatory properties of 3-(hydroxymethyl)-6,7-dihydroindolo[2,3-a]quinolizin-(12H)-one in RAW 264.7 murine macrophages. The results indicated that it inhibited the overproduction of NO and the release of TNF-α. Furthermore, this compound inhibited the expression of iNOS and COX-2 proteins, the enzymatic activity of iNOS, and the translocation of NF-κB to the nucleus induced by LPS. Therefore, we suggested that the effect of 3-(hydroxymethyl)-6,7-dihydroindolo[2,3-a]quinolizin-(12H)-one-mediated inhibition of the expression of LPS-induced iNOS and COX-2 genes is due to the suppression of NF-κB activation in the transcriptional level.

Key words

Nauclea officinalis - Rubiaceae - indole alkaloid - anti-inflammatory effect

Full Text

References

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