Liquid Chromatography Tandem Mass Spectrometry Method for Determination of Antidiabetic Chalcones Derivative S001-469 in Rat Plasma, Urine and Feces: Application to Pharmacokinetic Study^[*]

Abstract

A sensitive and selective liquid chromatography tandem mass spectrometry assay was developed for quantitation of a novel antidiabetic chalcones derivative S001-469 in rat matrices. Plasma and urine samples were prepared by double liquid-liquid extraction with diethyl ether and feces by protein precipitation using acetonitrile. Chromatographic elution was carried on cyano guard column (30 mm×4.6 mm i.d., 5 µm) in isocratic mode at a flow rate of 0.75 mL/min using mobile phase comprising of methanol: ammonium acetate buffer (pH 4.6, 10 mM) (90:10, v/v). Run time was 6 min. Detection was achieved by employing positive ionization mode on a triple-quadrupole LC–MS/MS system with an electrospray ionization (ESI) source. The calibration curves were linear over the range of 0.78–400 ng/mL for all 3 matrices. The method was validated and proved reliable through high and consistent intra- and inter- day accuracy and precision (<15%) values. Recoveries was >85% from spiked plasma, urine and feces samples. S001–469 was stable in plasma at room temperature till 8 h and at −60°C for 30 d and 3 freeze-thaw cycles.

Key words

antidiabetic - LC-MS/MS - pharmacokinetics - rat plasma - S001-469

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