Simultaneous Determination of Five Minor Coumarins and Flavonoids in
                        Glycyrrhiza uralensis by Solid-Phase Extraction and High-Performance
                    Liquid Chromatography/Electrospray Ionization Tandem Mass
                    Spectrometry

Xue Qiao; Chun-Fang Liu; Shuai Ji; Xiong-Hao Lin; De-An Guo; Min Ye

doi:10.1055/s-0033-1360272

Planta Medica, Table of Contents

Planta Med 2014; 80(02/03): 237-242
DOI: 10.1055/s-0033-1360272

Analytical Studies

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Simultaneous Determination of Five Minor Coumarins and Flavonoids in Glycyrrhiza uralensis by Solid-Phase Extraction and High-Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry

Xue Qiao^*

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China

,

Chun-Fang Liu^*

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China

,

Shuai Ji

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China

,

Xiong-Hao Lin

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China

,

De-An Guo

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China

,

Min Ye

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China

› Author Affiliations

Abstract

Minor phenolic compounds in licorice (Glycyrrhiza uralensis) have recently been proved for diverse bioactivities and favorable bioavailability, indicating that they may play an important role in the therapeutic effects or herb-drug interactions of licorice. However, so far, their abundance in licorice remains unknown. In this study, a reliable solid-phase extraction coupled with a high-performance liquid chromatography and diode array detection method was established to determine the minor phenolic compounds in licorice. The analytes were enriched by a three-step solid-phase extraction method, and then separated on a YMC ODS-A column by gradient elution. Five coumarins and flavonoids were identified by electrospray ionization tandem mass spectrometry, and then quantified using high-performance liquid chromatography and diode array detection. The amounts of glycycoumarin, dehydroglyasperin C, glycyrol, licoflavonol, and glycyrin in G. uralensis were 0.81 ± 0.28, 1.25 ± 0.59, 0.20 ± 0.08, 0.12 ± 0.04, and 0.17 ± 0.08 mg/g, respectively. Abundances of these compounds in other Glycyrrhiza species (G. glabra, G. inflata, and G. yunnanesis) were remarkably lower than G. uralensis.

Key words

Glycyrrhiza uralensis - Leguminosae - licorice - SPE-HPLC-DAD - coumarins - flavonoids

Full Text

References

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