Physical Exercise Introduced After Weaning Enhances Pancreatic Islet Responsiveness to Glucose and Potentiating Agents in Adult MSG-Obese Rats

R. A. Ribeiro; M. L. Bonfleur; E. C. Vanzela; A. I. Zotti; D. X. Scomparin; A. C. Boschero; S. L. Balbo

doi:10.1055/s-0034-1368705

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 2014; 46(09): 609-614
DOI: 10.1055/s-0034-1368705

Endocrine Research

Physical Exercise Introduced After Weaning Enhances Pancreatic Islet Responsiveness to Glucose and Potentiating Agents in Adult MSG-Obese Rats

R. A. Ribeiro

¹Universidade Federal do Rio de Janeiro (UFRJ), Campus UFRJ-Macaé, Macaé, RJ, Brazil

,

M. L. Bonfleur

²Laboratório de Fisiologia Endócrina e Metabolismo, Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná (UNIOESTE), Cascavel, PR, Brazil

,

E. C. Vanzela

³Laboratório de Pâncreas Endócrino e Metabolismo, Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil

,

A. I. Zotti

²Laboratório de Fisiologia Endócrina e Metabolismo, Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná (UNIOESTE), Cascavel, PR, Brazil

,

D. X. Scomparin

⁴Laboratório de Biologia Celular da Secreção, Departamento de Biologia Celular e Genética, Universidade Estadual de Maringá (UEM), Maringá, PR, Brazil

,

A. C. Boschero

³Laboratório de Pâncreas Endócrino e Metabolismo, Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Campinas, SP, Brazil

,

S. L. Balbo

²Laboratório de Fisiologia Endócrina e Metabolismo, Centro de Ciências Biológicas e da Saúde, Universidade Estadual do Oeste do Paraná (UNIOESTE), Cascavel, PR, Brazil

› Institutsangaben

Abstract

Physical exercise represents an alternative way to prevent and/or ameliorate chronic metabolic diseases. Disruption of sympathetic nervous system (SNS) activity contributes to adiposity in obese subjects. Here, we verified the preventive effect of swimming training upon adiposity, adrenal catecholamine storage, and pancreatic islet function in obese monosodium glutamate (MSG)-treated rats. Male neonatal Wistar rats received MSG (4 mg/g body weight) during the first 5 days of life and, at weaning, half of the rats were submitted to swimming training, 30 min/day, 3 days a week, until 90 days of age (exercised rats: MSGex). Half of the rats were used as controls (sedentary group, MSGsd). Exercise training (ET) decreased insulinemia and fat deposition in MSGex, and increased adrenal catecholamine content, compared with MSGsd rats. Insulinemia during the ivGTT was lower in MSGex rats, despite a lack of difference in glycemia. Swimming training enhanced insulin release in islets challenged by 2.8–8.3 mmol/l glucose, whereas, at supraphysiological glucose concentrations (11.1–16.7 mmol/l), MSGex islets secreted less insulin than MSGsd. No differences in insulin secretion were observed following l-arginine (Arg) or K⁺ stimuli. In contrast, islets from MSGex rats secreted more insulin when exposed to carbachol (100 μmol/l), forskolin (10 μmol/l), or IBMX (1 mmol/l) at 8.3 mmol/l glucose. Additionally, MSGex islets presented a better epinephrine inhibition upon insulin release. These results demonstrate that ET prevented the onset of obesity in MSG rats, probably by enhancing adrenal catecholamine levels. ET ameliorates islet responsiveness to several compounds, as well as insulin peripheral action.

Key words

adrenal catecholamine - exercise training - insulin secretion - MSG-obese rats - swimming training

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Referenzen

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