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Horm Metab Res 2014; 46(08): 561-567
DOI: 10.1055/s-0034-1370992
Endocrine Research
© Georg Thieme Verlag KG Stuttgart · New York

Relationship of Angiotensinase and Vasopressinase Activities Between Hypothalamus, Heart, and Plasma in L-NAME-Treated WKY and SHR

A. B. Villarejo
1   Unit of Physiology, Department of Health Sciences, University of Jaén, Spain
,
I. Prieto
1   Unit of Physiology, Department of Health Sciences, University of Jaén, Spain
,
A. B. Segarra
1   Unit of Physiology, Department of Health Sciences, University of Jaén, Spain
,
I. Banegas
1   Unit of Physiology, Department of Health Sciences, University of Jaén, Spain
,
R. Wangensteen
1   Unit of Physiology, Department of Health Sciences, University of Jaén, Spain
,
F. Vives
2   Instituto de Neurociencia ‘Federico Oloriz’, University of Granada, Granada, Spain
,
M. de Gasparo
3   Cardiovascular & Metabolic Syndrome Adviser, Rossemaison, Switzerland
,
M. Ramírez-Sánchez
1   Unit of Physiology, Department of Health Sciences, University of Jaén, Spain
› Author Affiliations
Further Information

Publication History

received 21 November 2013

accepted 17 February 2014

Publication Date:
13 March 2014 (online)

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Abstract

The renin-angiotensin system (RAS), vasopressin, and nitric oxide (NO) interact to regulate blood pressure at central and peripheral level. To improve our understanding of their interaction and their relationship with the hypothalamus and the cardiovascular system, we analyzed angiotensin- and vasopressin-metabolizing activities in hypothalamus (HT), left ventricle (LV), and plasma, collected from Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR) treated or not with L-NAME [N(G)-nitro-l-arginine methyl ester], which inhibits the formation of NO and over-activates the sympathetic nervous system. Previous observations in WKY suggested higher formation of Ang III and Ang IV in the HT and higher availability of Ang II in plasma after L-NAME treatment. Our current results show higher formation of Ang IV and higher metabolism of vasopressin after treatment with L-NAME in the LV of WKY rats. In SHR treated with L-NAME, there is higher availability of Ang III in the HT leading to higher release of vasopressin together with lower formation of Ang 2–10. In their LV, however, there is an increase of vasopressinase. Interestingly, while the enzymatic activities in the HT and LV of WKY rats and control SHR are poorly correlated, they are well but inversely correlated in the L-NAME treated SHR. On the other hand, no significant correlations between enzymatic activities in HT or LV and plasma were noticed. Our results suggest that eNOS inhibition in SHR induces or enhances an inverse reciprocal interaction between HT and LV involving the RAS and vasopressin, which may be mediated by the autonomic nervous system.

Key words

brain-heart connection - renin-angiotensin system - vasopressin - autonomic nervous system
 

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