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Planta Med 2015; 81(17): 1597-1603
DOI: 10.1055/s-0034-1396202
Mini Reviews
Georg Thieme Verlag KG Stuttgart · New York

Different Ways to On-Line Hyphenate Centrifugal Partition Chromatography and Mass Spectrometry: Application to Prenylated Xanthones from Garcinia mangostana

Emilie Destandau
1   ICOA, Univ-Orleans, CNRS, UMR 7311, Orleans, France
,
Thomas Michel
2   Université Nice Sophia Antipolis, UMR 7272 CNRS, Institut de Chimie de Nice, Parc Valrose, Nice, France
,
Alix Toribio
3   Département Actifs, Direction Recherche & Technologie, Chanel Parfums Beauté, Pantin, France
,
Claire Elfakir
1   ICOA, Univ-Orleans, CNRS, UMR 7311, Orleans, France
› Author Affiliations
Further Information

Publication History

received 03 October 2014

accepted 04 December 2014

Publication Date:
23 January 2015 (online)

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Erratum for: Different Ways to On-Line Hyphenate Centrifugal Partition Chromatography and Mass Spectrometry: Application to Prenylated Xanthones from Garcinia mangostanaPlanta Med 2015; 81(17): E5-E5
DOI: 10.1055/s-0035-1545712

Abstract

Centrifugal partition chromatography is a liquid-liquid separation method well adapted for the fractionation or purification of natural compounds from plant extracts. However, following the preparative isolation, the fractions collected must be analysed by high-performance thin-layer chromatography or high-performance liquid chromatography to evaluate their composition and/or their purity. These additional steps are time-consuming and increase the risk of compound degradation. In order to get an instantaneous analysis of fraction content with structural information on the phytochemicals eluted, it is possible to hyphenate on-line centrifugal partition chromatography with mass spectrometry. Depending on the complexity of the extract, two different kinds of centrifugal partition chromatography-mass spectrometry coupling can be performed: centrifugal partition chromatography-mass spectrometry or centrifugal partition chromatography-high-performance liquid chromatography-mass spectrometry coupling. In the first case, one part of the centrifugal partition chromatography effluent is directly introduced in the mass spectrometry ionisation source to identify the eluted compounds, while in the second case, it is directed to a high-performance liquid chromatography-mass spectrometry system where compounds are first separated thanks to high-performance liquid chromatography and then identified using mass spectrometry.

Key words

Garcinia mangostana - Clusiaceae - mangosteen - centrifugal partition chromatography - mass spectrometry - variable flow splitter - prenylated xanthones
 

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