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DOI: 10.1055/s-0035-1558111
Development of a Sustainable Procedure for the Recovery of Hydroxytyrosol from Table Olive Processing Wastewater Using Adsorption Resin Technology and Centrifugal Partition Chromatography
Publication History
received 07 October 2014
revised 01 June 2015
accepted 03 September 2015
Publication Date:
13 October 2015 (online)
![](https://www.thieme-connect.de/media/plantamedica/201517/lookinside/thumbnails/10-1055-s-0035-1558111_pmy1134-1.jpg)
Abstract
The present endeavor aims to establish a novel procedure, applicable to the extraction and isolation of hydroxytyrosol from table olive processing wastewater. A two-step chromatographic separation is presented using non-ionic absorbent resin for the recovery of its phenolic content, followed by purification of hydroxytyrosol with centrifugal partition chromatography. Two table olive processing wastewaters, obtained from Kalamon and Amfissis olive varieties, were used. In the extracts obtained after resin treatment, the hydroxytyrosol content was determined by high-performance liquid chromatography with diode-array detection to be 4.05 % and 10.10 %, respectively. The extract from Amfissis table olive processing wastewater was further processed with preparative centrifugal partition chromatography for the purification of hydroxytyrosol. High-performance liquid chromatography analysis revealed that the isolated compound was > 95 % purity.
Key words
adsorption resin - countercurrent chromatography - centrifugal partition chromatography - hydroxytyrosol - table olive processing wastewater-
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