Screening for Anti-allergic Activity of Natural Products

M Korinek; M El-Shazly; YC Tsai; LC Wang; ML Yu; YC Wu; BH Chen; FR Chang

doi:10.1055/s-0037-1608097

Planta Medica International Open, Table of Contents

Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608097

Poster Session

Georg Thieme Verlag KG Stuttgart · New York

Screening for Anti-allergic Activity of Natural Products

M Korinek

¹Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan

²Department of Biotechnology, College of Life Science, Kaohsiung Medical University, Kaohsiung, Taiwan

,

M El-Shazly

¹Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan

³Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Ain-Shams University, Cairo, Egypt

,

YC Tsai

¹Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan

,

LC Wang

¹Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan

,

ML Yu

¹Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan

,

YC Wu

¹Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan

⁴Research Center for Natural Products & Drug Development, Kaohsiung Medical University, Kaohsiung, Taiwan

,

BH Chen

²Department of Biotechnology, College of Life Science, Kaohsiung Medical University, Kaohsiung, Taiwan

⁵Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

,

FR Chang

¹Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan

⁶Research Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

› Author Affiliations

Abstract

Full Text

Increasing prevalence of allergic diseases with inadequate variety of treatment drives forward search for new alternative drugs. The untapped potential of natural products as an inexhaustible mine of biologically active compounds encouraged us to investigate the potential of separating natural products with promising anti-allergic activity. We utilized degranulation assay [1], which is based on β-hexosaminidase release from RBL-2H3 cells activated either by calcium ionophore A23187 or antigen (IgE plus DNP-BSA). Over 200 samples were screened for anti-allergic activity revealing a potential of several plants. For instance, ornamental plant Dietes bicolor, seeds of Aquilaria malaccensis, aerial parts of Pandanus amaryllifolius and Liriope platyphylla, leaves of Carica papaya, Artabotrys vinhensis, or nonpolar extracts of Typhonium blumei and T. roxburghii [2] exerted promising anti-allergic activity. The fractionation of these extracts, aiming to purify and identify the active compound, yielded various results, with each tested material developing its own unique story. For example, pursuing bioactivity-guided fractionation, anti-allergic phorbol ester was isolated from the seeds of Aquilaria malaccensis [3]. In contrast, the component of active fraction of Liriope platyphylla was never identified, while the ethanol layer of Carica papaya leaves was much more active than any of the isolated pure compounds. Interestingly, some of the chlorophyll-rich fractions showed potent activity, however, the toxicity of the samples raised upon light exposure, thus limiting their utilization. The active compound or mixture may serve as a new candidate for further anti-allergic evaluation and mechanism of action study.

Fig. 1

[1] Chen BH, Wu PY, Chen KM, Fu TF, Wang HM, Chen CY. J Nat Prod 2009; 72: 950 – 953.

[2] Korinek M, Wagh VD, Lo IW, Hsu YM, Hsu HY, Hwang TL, et al. Int J Mol Sci 2016; 17: 398.

[3] Korinek M, Chen KM, Jiang YH, El-Shazly M, Stocker J, Chou CK, et al. Phytomedicine 2016; 23: 1706 – 1715.

Figures

Fig. 1