Anti-inflammatory and anti-tubercular properties screening of natural products from Plectranthus species

J Marçalo; C Garcia; L Custódio; M Nicolai; C Reis; J Rodrigues Maria; A Romagnoli; E Petruccioli; D Goletti; L Monteiro Rodrigues; C Faustino; M Fimia Gian; P Rijo

doi:10.1055/s-0037-1608136

Planta Medica International Open, Inhaltsverzeichnis

Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608136

Poster Session

Georg Thieme Verlag KG Stuttgart · New York

Anti-inflammatory and anti-tubercular properties screening of natural products from Plectranthus species

J Marçalo

¹Universidade Lusófona's Research Center for Biosciences and Health Technologies (CBIOS), Lisboa, Portugal

,

C Garcia

¹Universidade Lusófona's Research Center for Biosciences and Health Technologies (CBIOS), Lisboa, Portugal

,

L Custódio

²Centre for Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, Faro, Portugal

,

M Nicolai

¹Universidade Lusófona's Research Center for Biosciences and Health Technologies (CBIOS), Lisboa, Portugal

,

C Reis

¹Universidade Lusófona's Research Center for Biosciences and Health Technologies (CBIOS), Lisboa, Portugal

³Biophysics and Biomedical Engineering Institute (IBEB), Faculty of Sciences, University of Lisbon (ULisboa), Lisbon, Portugal

,

J Rodrigues Maria

²Centre for Marine Sciences (CCMAR), University of Algarve, Campus of Gambelas, Faro, Portugal

,

A Romagnoli

⁴National Institute for the Infectious Diseases “Lazzaro Spallanzani”, Rome, Italy

,

E Petruccioli

⁴National Institute for the Infectious Diseases “Lazzaro Spallanzani”, Rome, Italy

,

D Goletti

⁴National Institute for the Infectious Diseases “Lazzaro Spallanzani”, Rome, Italy

,

L Monteiro Rodrigues

¹Universidade Lusófona's Research Center for Biosciences and Health Technologies (CBIOS), Lisboa, Portugal

,

C Faustino

⁵Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon (ULisboa), Lisbon, Portugal

,

M Fimia Gian

⁴National Institute for the Infectious Diseases “Lazzaro Spallanzani”, Rome, Italy

⁶Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Lecce, Italy

,

P Rijo

¹Universidade Lusófona's Research Center for Biosciences and Health Technologies (CBIOS), Lisboa, Portugal

⁵Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, University of Lisbon (ULisboa), Lisbon, Portugal

› Institutsangaben

Abstract

Volltext

Medicinal plants of the Plectranthus genus are widely used in traditional medicine for their anti-inflammatory and anti-infective properties [1]. Previous reports [2,3] directed this study for the production and release of Nitric Oxide, and for the CFU analysis of Mycobacterium tuberculosis H37Rv (Mtb) growth inhibition, of Plectranthus spp. non-cytotoxic compounds.

The natural compounds isolated from Plectranthus spp. using bioassay-guided fractionation of extracts, were: 6β,7α-dihydroxyroyleanone and 6,7-dehydroroyleanone (P. madagascariensis); β-sitosterol, stigmasterol, oleanolic and ursolic acids (P. ecklonii); (13S,15S)-6β,7α,12α,19-tetrahydroxy-13β,16-cyclo-8-abietene-11,14-dione (P. porcatus); (11R*,13E)-11-acetoxyhalima-5,13-dien-15-oic acid, Plectrornatin C, 1,6-di-O-acetylforskolin and 1,6-di-O-acetyl-9-deoxyforskolin (P. ornatus); α-amyrin and β-amyrin (P. neochilus); chlorogenic acid (P. saccatus) and rosmarinic acid (all aqueous extracts).

The compounds were unable to reveal in vitro anti-inflammatory activity neither in LPS-stimulated RAW 264.7 cells nor in the S-nitroso-N-acetylpenicillamine assay. Nonetheless, regarding the Mtb growth inhibition, one semi-synthetic halimane derivative (11R*,13E)-halima-5,13-diene-11,15-diol) previously prepared [4], revealed promising results (2.1 × 10⁵ CFU/mL), with an effect similar to the anti-tubercular drugs ethambutol (2.0 × 10⁵ CFU/mL) and isoniazid (1.2 × 10⁵ CFU/mL).

To the best of our knowledge, this report is the first scientific validation on Plectranthus spp. isolated compounds concerning their anti-inflammatory activity for NO production and release, and concerning their anti-mycobacterial activity for Mtb growth inhibition. Further studies regarding other inflammatory mediators are under study.

[1] Lukhoba CW, Simmonds MS, Paton AJ. J. Ethnopharmacol 2006; 103: 1 – 24;

[2] Rijo, P, Simões MF, Francisco AP, Rojas R, Gilman RH, Vaisberg AJ, Rodríguez B, Moiteiro C. Chem. Biodivers 2010; 7: 922 – 932;

[3] Rijo, P, Gaspar-Marques C, Simões MF, Jimeno ML, Rodríguez B. Biochemical Systematics and Ecology 2007; 35: 215 – 221.