Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608355
Poster Session
Georg Thieme Verlag KG Stuttgart · New York

Elicitation of Bioactive Compounds by Yeast Extract in Shoot Culture of Dioscorea birmanica Prain & Burkill

Y Jirakiattikul
1   Division of Agricultural Technology, Faculty of Science and Technology, Thammasat University, Rangsit campus, Pathumthani, Thailand
,
P Rithichai
1   Division of Agricultural Technology, Faculty of Science and Technology, Thammasat University, Rangsit campus, Pathumthani, Thailand
,
R Jirapongpatta
1   Division of Agricultural Technology, Faculty of Science and Technology, Thammasat University, Rangsit campus, Pathumthani, Thailand
,
A Itharat
2   Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Rangsit campus, Pathumthani, Thailand
› Author Affiliations
Further Information

Publication History

Publication Date:
24 October 2017 (online)

 
 

    Dioscorea birmanica Prain & Burkill is a medicinal plant which its rhizome has been used with other medicinal plants for the treatment of cancers, septicemia diseases and AIDS [1]. It has been reported that the regenerated shoots of D. birmanica can synthesize and accumulate lower amount of bioactive compounds than those of mother rhizomes [2]. Therefore, the enhancement of bioactive compounds in the regenerated shoots by elicitation with yeast extract (YE) at different times of culture period was investigated. The 6-weeks old regenerated shoots were cultured on MS medium supplemented with or without 3 g/l YE for 1, 2, 4 and 6 weeks. It was indicated that the shoots cultured on medium supplemented with 3 g/l YE for 2 weeks contained the highest total saponin content of 1.23 ± 0.05 mg diosgenin/g DW or 1.93 times higher than that of the non-YE shoots cultured for 2 weeks. However, YE had no effect on total phenolic content and DPPH radical scavenging activity of D. birmanica shoots cultured under aseptic conditions.

    [1] Tewtrakul S, Itharat A. Bioorg Med Chem 2006; 14: 8707 – 8711

    [2] Jirakiattikul Y, Rithichai P, Songsri O, Ruangnoo S, Itharat A. Acta Physiol Plant 2016; 38: 249.


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