INFLUENCE OF CO-ADMINISTRATION OF ARTEMISININ AND RICINODENDRON HEUDELOTII LEAF EXTRACT ON ANTIOXIDANTS AND LIVER FUNCTION IN MALE RATS

O Yakubu; A Adebayo; E Okechukwu; O Adeyemi; E Iweala; YJ Zhang

doi:10.1055/s-0037-1608442

Planta Medica International Open, Table of Contents

Planta Medica International Open 2017; 4(S 01): S1-S202
DOI: 10.1055/s-0037-1608442

Poster Session

Georg Thieme Verlag KG Stuttgart · New York

INFLUENCE OF CO-ADMINISTRATION OF ARTEMISININ AND RICINODENDRON HEUDELOTII LEAF EXTRACT ON ANTIOXIDANTS AND LIVER FUNCTION IN MALE RATS

O Yakubu

¹1Biochemistry and Molecular Biology Unit, Department of Biological Sciences, College of Science and Technology, Covenant University, PMB 1023, Canaan land, Ota Ogun State, Nigeria

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A Adebayo

¹1Biochemistry and Molecular Biology Unit, Department of Biological Sciences, College of Science and Technology, Covenant University, PMB 1023, Canaan land, Ota Ogun State, Nigeria

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E Okechukwu

¹1Biochemistry and Molecular Biology Unit, Department of Biological Sciences, College of Science and Technology, Covenant University, PMB 1023, Canaan land, Ota Ogun State, Nigeria

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O Adeyemi

¹1Biochemistry and Molecular Biology Unit, Department of Biological Sciences, College of Science and Technology, Covenant University, PMB 1023, Canaan land, Ota Ogun State, Nigeria

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E Iweala

¹1Biochemistry and Molecular Biology Unit, Department of Biological Sciences, College of Science and Technology, Covenant University, PMB 1023, Canaan land, Ota Ogun State, Nigeria

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YJ Zhang

²State Key Laboratory of Phytochemistry and Plant Resources of West China, Kunming Institute of Botany, #132, Lanhei Road, Heilongtan, Kunming China, Kunming, Nigeria

› Author Affiliations

Abstract

Full Text

Startling rate of malaria parasite resistance to Artemisinin and its derivatives has led to possible herb-drug combination therapy¹. This study assessed the effect of co-administration of artemisinin and Ricinodendron heudelotii leaf extract on certain antioxidants and liver parameters in rats and also phytochemical analysis of different fractions of the extract. 40 male rats were randomly grouped into four and administered: distilled water (Group A), artemisinin only (Group B), artemisinin with R. heudelotii extract (Group C) and R. heudelotii extract only (Group D). Alanine amino transferase (ALT), Aspartate aminotransferase, Alkaline phosphatase (ALP). Total protein were determined using standard methods. Reduced glutathione (GSH), catalase and malondialdehyde (MDA) were determined spectrophotometrically while the qualitative and quantitative phytochemical analysis was also carried out. The results revealed that total protein increased significantly in group C. The activities of ALT and AST were significantly increased (p ≤0.05) in the group administered artemisinin only while their activities were regulated to control level both in group C and D. The liver GSH concentration decreased in the group administered artemisinin only compared to the other treatment groups. Similarly, group A exhibited significant increase (p ≤0.05) in MDA while group C and D showed decrease in MDA and catalase concentration. Histological examination showed that few hepatocytes were necrotic in the group administered artemisinin only while group C only showed mild to moderate central venous congestion and periportal cellular infiltration. The study indicates that the bioactive constituents in the R. heudelotii extract might have a regulatory effect on artemisinin toxicity or collaboratively enhance its activity. Such bioconstituents can further be isolated and characterized for drug development to tackle Plasmodium falciparum resistance.

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