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Thromb Haemost 2002; 87(06): 1043-1050
DOI: 10.1055/s-0037-1613130
Review Article
Schattauer GmbH

FAK-mediated Inhibition of Vascular Smooth Muscle Cell Migration by the Tetraspanin CD9

Arnaud Scherberich   *
,
Grégory Giannone
1   INSERM U268, Hôpital Paul Brousse, Villejuif, France
,
Elisabeth Perennou
1   INSERM U268, Hôpital Paul Brousse, Villejuif, France
,
Kenneth Takeda
1   INSERM U268, Hôpital Paul Brousse, Villejuif, France
,
Claude Boucheix
1   INSERM U268, Hôpital Paul Brousse, Villejuif, France
,
Eric Rubinstein
1   INSERM U268, Hôpital Paul Brousse, Villejuif, France
,
François Lanza
2   INSERM U311, Etablissement de Transfusion Sanguine, Strasbourg, France
,
Alain Beretz
1   INSERM U268, Hôpital Paul Brousse, Villejuif, France
› Author Affiliations
Further Information

Publication History

Received 08 October 2001

Accepted after resubmission 11 March 2002

Publication Date:
08 December 2017 (online)

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Summary

Migration of vascular smooth muscle cells (SMC) towards the intima is a key event in vascular proliferative diseases. We investigated a potential role for the tetraspanin CD9 in this process in a wound migration assay. Aortic SMC from CD9 knock-out mice had higher migration rates and the presumably stimulatory anti-CD9 antibody ALMA-1 inhibited migration of human SMC. The signaling pathways responsible for this inhibitory effect were investigated. In migrating CD9−/− SMC, stress fiber formation was decreased and focal adhesions were smaller and more diffusely distributed, consistent with an inhibition of integrin clustering. In migrating mouse SMC expressing CD9, focal adhesion kinase (FAK) tyrosine phosphorylation was doubled. No differences in intracellular calcium signaling were observed between CD9+/+ and CD9−/− SMC during migration. We suggest that CD9 inhibits SMC migration by a stimulation of both stress fiber formation and integrin clustering, leading to a stimulation of FAK phosphorylation.

Keywords

CD9 - tetraspanin - cell migration - smooth muscle - integrin - FAK - collagen

* Present address: Friedrich Miescher Institute for Biomedical Research, Maulbeerstraße 66, CH-4058 Basel, Switzerland


 

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