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Thromb Haemost 2003; 90(06): 1214-1215
DOI: 10.1055/s-0037-1613426
Letters to the Editor
Schattauer GmbH

In vitro high level protein S expression after modification of protein S cDNA

Suely M. Rezende
,
Cristina Razzari
,
Rachel E. Simmonds
Financial support: This work was supported by a grant from the Brazilian Government (Agencia CAPES) and grants from the British Heart Foundation.
Further Information

Publication History

Received 30 May 2003

Accepted after revision 29 July 2003

Publication Date:
09 December 2017 (online)

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  • References

  • 1 Malm J, Cohen E, Dackowski W. et al. Expression of completely gamma-carboxylated and beta-hydroxylated recombinant human vitamin-K-depen-dentproteinS with full biological activity. Eur J Biochem 1990; 187: 737-43.
    CrossrefPubMedSearch in Google Scholar
  • 2 Giri TK, de Frutos PG, Dahlback B. Protein S Thrl03 Asn mutation associated with type II deficiency reproduced in vitro and functionally characterised. Thromb Haemost 2000; 84: 413-9.
    Thieme ConnectPubMedSearch in Google Scholar
  • 3 Kozak M. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 1986; 44: 283-92.
    CrossrefPubMedSearch in Google Scholar
  • 4 Kaufman RJ. Vectors used for expression in mammalian cells. Methods in Enzymology 1990; 185: 487-511.
    PubMedSearch in Google Scholar
  • 5 Rezende SM, Lane DA, Zoller B. et al. Genetic and phenotypic variability between families with hereditary protein S deficiency. Thromb Haemost 2002; 87: 258-65.
    Thieme ConnectPubMedSearch in Google Scholar
  • 6 Rezende SM, Lane DA, Mille-Baker B. et al. Protein S Gla-Domain Mutations Causing Impaired Ca2+-Induced Phospholipid Binding and Severe Functional Deficiency. Blood 2002; 100: 2812-9.
    CrossrefPubMedSearch in Google Scholar
  • 7 Stenberg Y, Drakenberg T, Dahlback B. et al. Characterization of recombinant epidermal growth factor (EGF)-like modules from vitamin-K-de-pendent protein S expressed in Spodoptera cells—the cofactor activity depends on the N-terminal EGF module in human protein S. Eur J Biochem 1998; 251: 558-64.
    CrossrefPubMedSearch in Google Scholar