Randomized, Placebo-controlled, Blinded and Cross-matched Study on the Antiplatelet Effect of Inhaled Nitric Oxide in Healthy Volunteers

André Gries; Axel Herr; Johann Motsch; Alexandra Holzmann; Jörg Weimann; Friedemann Taut; Natalie Erbe; Christoph Bode; Eike Martin

doi:10.1055/s-0037-1613804

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2000; 83(02): 309-315
DOI: 10.1055/s-0037-1613804

Rapid Communication

Schattauer GmbH

Randomized, Placebo-controlled, Blinded and Cross-matched Study on the Antiplatelet Effect of Inhaled Nitric Oxide in Healthy Volunteers

André Gries

,

Axel Herr

,

Johann Motsch

,

Alexandra Holzmann

,

Jörg Weimann

,

Friedemann Taut

,

Natalie Erbe

¹German Cancer Research Center, University of Heidelberg, Germany

,

Christoph Bode

²Department of Cardiology, University of Freiburg, Germany

,

Eike Martin

› Author Affiliations

Abstract

Summary

The platelet inhibitory effect of 0-40 ppm inhaled nitric oxide (NO) was investigated in healthy men and women. In both groups, ADPand collagen-induced platelet aggregation was significantly inhibited 20 (T20) and 40 min (T40) after the beginning of inhalation of 5, 10, and 40 ppm. Moreover, in both men and women, the in vitro bleeding time was significantly prolonged at T20 and T40 during inhalation of 40 ppm. Inhalation of NO also inhibited P-selectin expression at 5, 10, and 40 ppm and fibrinogen binding to the GPIIb/IIIa-receptor at 40 ppm. In conclusion, in healthy volunteers, the platelet inhibitory effect of inhaled NO was not dose-related, since it was significant at 5 and 10 ppm but did not increase during the administration of higher NO concentrations. In addition, gender-related differences were only observed in ADP-induced platelet aggregation at 10 ppm and in bleeding time prolongation at 40 ppm.

Keywords

Platelets - aggregation - bleeding time - P-selectin expression - fibrinogen binding - inhaled nitric oxide

Full Text

References

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