Automated, Simultaneous Detection of the Factor V Leiden and Prothrombin (G20210A) Variants Using Multiplex PCR and a Line Probe Assay

A. Leyte; P.H. M. Smits; J.P. van Straalen; L.J. van Doorn; W.G.V. Quint

doi:10.1055/s-0037-1613818

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2000; 83(02): 354-355
DOI: 10.1055/s-0037-1613818

Letters to the Editor

Schattauer GmbH

Automated, Simultaneous Detection of the Factor V Leiden and Prothrombin (G20210A) Variants Using Multiplex PCR and a Line Probe Assay

A. Leyte

¹From the 1Clinical Chemistry and Hematology Laboratory en Academical Medical Centre (AMC), Amsterdam

,

P.H. M. Smits

²Molecular Biology Laboratory, Slotervaart Hospital, Academical Medical Centre (AMC), Amsterdam

,

J.P. van Straalen

³Clinical Chemistry Laboratory (LAKC), Academical Medical Centre (AMC), Amsterdam

,

L.J. van Doorn

⁴Delft Diagnostic Laboratory (DDL), Delft, The Netherlands

,

W.G.V. Quint

⁴Delft Diagnostic Laboratory (DDL), Delft, The Netherlands

Refrences
1 Bertina RM, Koeleman BP, Koster T, Rosendaal FR, Dirven RJ, De Ronde H, van der Velden PA, Reitsma PH. Mutation in blood coagulation factor V associated with resistance to activated protein C. Nature 1994; 369: 64-7.
2 Poort SR, Rosendaal FR, Reitsma PH, Bertina RM. A common genetic variation in the 3’-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis. Blood 1996; 88: 3698-703.
3 Ripoll L, Paulin D, Thomas S, Dronet LO. Multiplex PCR-mediated sitedirected mutagenesis for one-step determination of factor V Leiden and G20210A transition of the prothrombin gene. Thromb Haemost 1997; 90: 960-1.
4 Gómez E, van der Poel SCPAM, Jansen J, van der Reijden BA, Löwenberg B. Rapid simultaneous screening of factor V Leiden and G20210A prothrombin variant by multiplex polymerase chain reaction on whole blood. Blood 1998; 91: 2208-9.
5 Meyer M, Kutcher G, Vogel G. Simultaneous genotyping for factor V Leiden by a multiplex PCR-SSCP assay on whole blood. Thromb Haemost 1999; 81: 162-3.
6 Keeney S, Salden A, Hay C, Cumming A. A whole blood, multiplex PCR detection method for factor V Leiden and the prothrombin G20210A variant. Thromb Haemost 1999; 81: 464-5.
7 Hézard N, Gornillet-Lefebre P, Gillot L, Potron G, Nguyen P. Multiplex ASA PCR for simultaneous determination of factor V Leiden gene, G→A 20210 prothrombin gene and C→T 677 MTHFR gene mutations. Thromb Haemost 1998; 79: 1054-5.
8 Stuyver L, Rossau R, Wyseur A, Duhamel B, Vandenborcht H, van Heuverswyn H, Maertens G. Typing of HCV isolates and characterization of new (sub)types using a line probe assay. J Gen Virol 1993; 74: 1093-102.
9 Van Doorn LJ, Figueiredo C, Sanna R, Pena AS, Midolo P, Ng EK, Atherton JC, Blaser MJ, Quint WGV. Expanding allelic diversity of Helicobacter pylori vacA. J Clin Microbiol 1998; 36: 2597-603.
10 Kleter B, van Doorn LJ, ter JSchegget, Schrauwen L, van Krimpen C, Burger MP, ter BHarmsel, Quint WGV. A novel short-fragment PCR assay for highly sensitive broad-spectrum detection of anogenital human papillomaviruses. Am J Pathol 1998; 153: 1731-9.
11 Scholte GHA, van Doorn LJ, Quint WGV, Lindeman J. Polymerase Chain Reaction for the detection of Helicobacter pylori in formaldehyde-sublimate fixed, paraffin-embedded gastric biopsies. Diagn Mol Pathol 1997; 06: 238-43.