Inhibition of Platelet Thrombosis Using an Activated Protein C-loaded Stent: In Vitro and In Vivo Results

Roger S.-Y. Foo; Anthony H. Gershlick; Kai Hogrefe; Julia H. Baron; Thomas W. Johnston; Amanda J. Hussey; Ian Garner; David P. de Bono

doi:10.1055/s-0037-1613843

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2000; 83(03): 496-502
DOI: 10.1055/s-0037-1613843

Review Article

Schattauer GmbH

Inhibition of Platelet Thrombosis Using an Activated Protein C-loaded Stent: In Vitro and In Vivo Results

Roger S.-Y. Foo

¹From the Division of Cardiology, Department of Medicine and Therapeutics, Leicester University

,

Anthony H. Gershlick

¹From the Division of Cardiology, Department of Medicine and Therapeutics, Leicester University

,

Kai Hogrefe

¹From the Division of Cardiology, Department of Medicine and Therapeutics, Leicester University

,

Julia H. Baron

¹From the Division of Cardiology, Department of Medicine and Therapeutics, Leicester University

,

Thomas W. Johnston

²From the PPL Therapeutics, Roslin, Edinburgh, UK

,

Amanda J. Hussey

²From the PPL Therapeutics, Roslin, Edinburgh, UK

,

Ian Garner

²From the PPL Therapeutics, Roslin, Edinburgh, UK

,

David P. de Bono

¹From the Division of Cardiology, Department of Medicine and Therapeutics, Leicester University

› Institutsangaben

Abstract

Summary

In high-risk and complicated coronary intervention, the risk of acute closure is unpredictable. Thrombus and platelet deposition at the intervention site may also have further effects on subsequent restenosis. In vivo infusion of activated protein C has previously been shown to achieve potent anticoagulation without any haemostatic side effects. We now evaluated the in vitro and in vivo efficacy of polymer-coated coronary stents loaded with purified rabbit Activated Protein C (APC). By measuring ¹²⁵I-fibrinogen/fibrin deposition APC-loaded stent-wires were antithrombotic compared to albumin-loaded, inhibited-APCloaded, plain polymer-coated and stainless steel stent-wires. In a balloon injury rabbit iliac artery model, APC-loaded stents did not occlude (0/14) compared to plain stents (9/15) and BSA-loaded stents (2/4). Relative ¹¹¹In-labelled platelet deposition showed a similarly significant degree of inhibition. In conclusion, APC-loading could render stents significantly less thrombotic. Whether an effective antithrombogenic stent like this effectively reduces restenosis rates warrants further evaluation.

Keywords

Activated protein C - stents - thrombosis - platelet deposition

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Referenzen

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