An Effective Route for the Isolation of Human Genetic Loci Exemplified Using the BglI Restriction Fragment Length Polymorphism of the Factor VIII Gene

• Refrences

• 1 Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anaemia. Science 1985; 230: 1350-4.
• 2 Gingrich JC, Boehrer DM, Garnes JA, Johnson W, Wong BS, Bergman A, Eveleth GG, Langlois RG, Carrano AV. Construction and characterisation of human chromosome-2 specific cosmid, fosmid and PAC clone libraries. Genomics 1996; 32: 65-74.
• 3 Bowen DJ, Hampton KK. Analysis of the BglI restriction fragment length polymorphism in the human factor VIII gene using “virtual PCR” - a novel approach employing the polymerase chain reaction in the absence of sequence information for the locus. Human Genetics 1996; 98: 219-22.
• 4 Antonarakis SE, Waber PG, Kittur SD, Patel AS, Kazazian Jr HH, Mellis MA, Counts RB, Stamatoyannopoulos G, Bowie EJ, Fas DN, Pittman DD, Wozney JM, Toole JJ. Haemophilia A: detection of molecular defects and of carriers by DNA analysis. New Engl J Med 1985; 313: 842-8.
• 5 Budowle B, Allen RC. Discontinuous polyacrylamide gel electrophoresis of DNA fragments. In: Protocols in human molecular genetics. Mathew CG. New Jersey: Humana Press; 1991: 128.