Influence of SIN-1 on Platelet Ca2+ Handling in Patients with Suspected Coronary Artery Disease: Ex Vivo and In Vitro Studies

Kim-Hanh Le Quan Sang; Claude Le Feuvre; Annie Brunet; Thuc Do Pham; Jean-Philippe Metzger; André Vacheron; Marie-Aude Devynck

doi:10.1055/s-0037-1613904

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2000; 83(05): 752-758
DOI: 10.1055/s-0037-1613904

Review Article

Schattauer GmbH

Influence of SIN-1 on Platelet Ca²⁺ Handling in Patients with Suspected Coronary Artery Disease: Ex Vivo and In Vitro Studies

Kim-Hanh Le Quan Sang

,

Claude Le Feuvre

¹Clinique Cardiologique, Hôpital Necker-Enfants Malades, Paris, France

,

Annie Brunet

,

Thuc Do Pham

,

Jean-Philippe Metzger

¹Clinique Cardiologique, Hôpital Necker-Enfants Malades, Paris, France

,

André Vacheron

¹Clinique Cardiologique, Hôpital Necker-Enfants Malades, Paris, France

,

Marie-Aude Devynck

› Institutsangaben

Abstract

Summary

The 3-morpholinosydnonimine (SIN-1) generates both nitric oxide (NO) and superoxide anion (O₂−). It elicits dose-dependent vasodilation in vivo, in spite of the opposite effects of its breakdown products on vascular tone and platelet aggregation.

This study was designed to investigate the influence of intravenous SIN-1 injection on platelet Ca²⁺ handling in patients undergoing coronary angiography. SIN-1 administration reduced cytosolic [Ca²⁺] in unstimulated platelets by decreasing Ca²⁺ influx. It attenuated Ca²⁺ mobilization from internal stores evoked by thrombin or thapsigargin.

In vitro studies were used as an approach to investigate how simultaneous productions of NO and O₂− from SIN-1 modify thrombin- or thapsigargin-induced platelet Ca²⁺ mobilization. Superoxide dismutase, the O₂− scavenger, enhanced the capacity of SIN-1 to inhibit Ca²⁺ mobilization but catalase had no effect.

This suggests that the effects of SIN-1 on platelet Ca²⁺ handling resemble those of NO, but are modulated by simultaneous O₂− release, independently of H₂O₂ formation.

Keywords

SIN-1 - nitric oxide - superoxide anion - platelet [Ca²⁺]_i - coronary artery disease

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Referenzen

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