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Thromb Haemost 1998; 79(01): 94-98
DOI: 10.1055/s-0037-1614227
Review Article
Schattauer GmbH

Rapid Detection of D-dimer Using a Fiber Optic Biosensor

Chris A. Rowe
1   Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, Washington, DC, USA
,
Jeni S. Bolitho
2   AGEN Biomedical Ltd., Acacia Ridge, QLD, Australia
,
Andrew Jane
2   AGEN Biomedical Ltd., Acacia Ridge, QLD, Australia
,
Peter G. Bundesen
2   AGEN Biomedical Ltd., Acacia Ridge, QLD, Australia
,
Dennis B. Rylatt
2   AGEN Biomedical Ltd., Acacia Ridge, QLD, Australia
,
Paul R. Eisenberg
3   Cardiovascular Division, Washington University School of Medicine, St. Louis, MO, USA
,
Frances S. Ligler
1   Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, Washington, DC, USA
› Author Affiliations
Further Information

Publication History

Received 08 April 1997

Accepted after resubmission 04 September 1997

Publication Date:
08 December 2017 (online)

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Summary

We describe a rapid and sensitive method for detection and quantification of D-dimer and other crosslinked fibrin degradation products (XL-FDPs), which are present in elevated concentrations in patients with sepsis and thrombotic disorders. The method utilizes a sandwich fluoroimmunoassay immobilized in the sensing region of an evanescent wave biosensor. Physiological concentrations of D-dimer and high molecular weight XL-FDP could be determined in buffer and plasma samples on calibrated fibers in 11 min. Samples from septic patients were assayed using ELISA and the fiber optic method; concentrations determined by fiber optic assay were strongly correlated with those determined by ELISA (r = 0.918); intra- and inter-assay errors were comparable to those from ELISAs. Given its accuracy and rapid response time, this fiber optic biosensor shows great potential for development as a diagnostic tool.

 

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