Comparative Study of Vanadate- and Phorbol Ester-induced Cyclo-oxygenase-2 Expression in Human Endothelial Cells

Kenzo Hirai; Yasuharu Ezumi; Eisuke Nishida; Takashi Uchiyama; Hiroshi Takayama

doi:10.1055/s-0037-1614869

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1999; 82(05): 1545-1561
DOI: 10.1055/s-0037-1614869

Rapid Communications

Schattauer GmbH

Comparative Study of Vanadate- and Phorbol Ester-induced Cyclo-oxygenase-2 Expression in Human Endothelial Cells

Kenzo Hirai

¹From the Department of Hematology and Oncology, Clinical Sciences for Pathological Organs, Graduate School of Medicine and Department of Biophysics, Graduate School of Science, Kyoto University, Japan

,

Yasuharu Ezumi

¹From the Department of Hematology and Oncology, Clinical Sciences for Pathological Organs, Graduate School of Medicine and Department of Biophysics, Graduate School of Science, Kyoto University, Japan

,

Eisuke Nishida

¹From the Department of Hematology and Oncology, Clinical Sciences for Pathological Organs, Graduate School of Medicine and Department of Biophysics, Graduate School of Science, Kyoto University, Japan

,

Takashi Uchiyama

¹From the Department of Hematology and Oncology, Clinical Sciences for Pathological Organs, Graduate School of Medicine and Department of Biophysics, Graduate School of Science, Kyoto University, Japan

,

Hiroshi Takayama

¹From the Department of Hematology and Oncology, Clinical Sciences for Pathological Organs, Graduate School of Medicine and Department of Biophysics, Graduate School of Science, Kyoto University, Japan

› Author Affiliations

Abstract

Summary

Our previous study showed that vanadate, an inhibitor of protein tyrosine phosphatases, induced the expression of cyclo-oxygenase (COX)-2 in a protein-tyrosine-kinase (PTK)-dependent manner in human umbilical vein endothelial cells (HUVEC). Here, we further compared the actions of vanadate and phorbol 12-myristate 13-acetate (PMA), an activator of protein kinase C (PKC), on induction of COX-2 with special reference to mitogen-activated protein kinases (MAPKs) in HUVEC. Vanadate induced activation of three families of MAPKs, extracellular signal-regulated kinases 1 and 2 (ERK1/2), p38, and c-Jun amino-terminal kinase (JNK) 1, while activation of ERK1/2 alone was induced by PMA. The former activation by vanadate and the latter one by PMA were inhibited by tyrphostin-47, an inhibitor of PTKs, and by Ro31-8220, a PKC inhibitor, respectively. Either tyrphostin-47, PD98059, a specific inhibitor of the upstream kinase toward ERK1/2, or SB203580, a specific inhibitor of p38, completely suppressed vanadate-induction of COX-2 mRNA and protein. On the other hand, PMA-induction of COX-2 mRNA and protein was abolished by Ro31-8220 or PD98059 but not by SB203580. These data indicate that PMA-induced and PKC-dependent expression of COX-2 requires mainly activation of ERK1/2 among MAPKs, while activation of both ERK1/2 and p38 or possibly of all three families of MAPKs is necessary for vanadate-induced and PTK-dependent expression of COX-2.

Key words

Cyclo-oxygenase - protein-tyrosine-kinase - protein kinase C - mitogen-activated protein kinase

Full Text

References

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