Endothelialization of Crosslinked Albumin-heparin Gels

Gert W. Bos; Nicole M. Scharenborg; André A. Poot; Gerard H. M. Engbers; Tom Beugeling; Willem G. van Aken; Jan Feijen

doi:10.1055/s-0037-1614910

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1999; 82(06): 1757-1763
DOI: 10.1055/s-0037-1614910

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Schattauer GmbH

Endothelialization of Crosslinked Albumin-heparin Gels

Gert W. Bos

¹From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands

,

Nicole M. Scharenborg

¹From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands

,

André A. Poot

¹From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands

,

Gerard H. M. Engbers

¹From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands

,

Tom Beugeling

¹From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands

,

Willem G. van Aken

¹From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands

,

Jan Feijen

¹From the Faculty of Chemical Technology, Section of Polymer Chemistry and Biomaterials and Institute for Biomedical Technology, University of Twente, Enschede, The Netherlands

› Author Affiliations

Abstract

Summary

Crosslinked gels of albumin as well as heparinized albumin gels, potential sealants of prosthetic vascular grafts, were studied with regard to in vitro stability, binding of basic fibroblast growth factor (bFGF) and cellular interactions. A small percentage of the heparin present in these gels, was released during storage in SDS solution. During storage in cell culture medium at 37° C, heparin release was 21-25 percent. Release of albumin did not occur.

Human umbilical vein endothelial cells (HUVECs) rapidly adhered and subsequently spread on (heparinized) albumin gels, but proliferation was only observed if heparin was present in the gel.

Binding of ¹²⁵I-bFGF to heparinized albumin gel was 35 percent higher than to non-heparinized albumin gel. Growth of HUVECs occurred only on heparinized albumin gel loaded with bFGF and not on bFGF-loaded albumin gel.

The number of platelets deposited under stationary conditions onto heparinized albumin gel was about twice the number found on nonheparinized albumin gel. Seeding of HUVECs on heparinized albumin gel, significantly reduced the number of platelets adhering to this surface. Moreover, no spreading of platelets was observed on substrates seeded with HUVECs.

It can be concluded that crosslinked gels of albumin to which heparin is immobilized, are candidate sealants for prosthetic vascular grafts and suitable substrates for endothelial cell seeding.

Keywords

Endothelialization - vascular prosthesis - bFGF - platelet deposition

Full Text

References

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