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Thromb Haemost 2001; 85(06): 1055-1059
DOI: 10.1055/s-0037-1615963
Review Article
Schattauer GmbH

The Role of Factor XI in a Dilute Thromboplastin Assay of Extrinsic Coagulation Pathway

Rong He
1   Thrombosis and Hemostasis Laboratory, Department of Physiology, Hunan Medical University, Changsha, Hunan, P. R. China
,
Shilong Xiong
1   Thrombosis and Hemostasis Laboratory, Department of Physiology, Hunan Medical University, Changsha, Hunan, P. R. China
,
Xiaofan He
1   Thrombosis and Hemostasis Laboratory, Department of Physiology, Hunan Medical University, Changsha, Hunan, P. R. China
,
Fayi Liu
1   Thrombosis and Hemostasis Laboratory, Department of Physiology, Hunan Medical University, Changsha, Hunan, P. R. China
,
Jianzhong Han
1   Thrombosis and Hemostasis Laboratory, Department of Physiology, Hunan Medical University, Changsha, Hunan, P. R. China
,
Juncheng Li
1   Thrombosis and Hemostasis Laboratory, Department of Physiology, Hunan Medical University, Changsha, Hunan, P. R. China
,
Shilin He
1   Thrombosis and Hemostasis Laboratory, Department of Physiology, Hunan Medical University, Changsha, Hunan, P. R. China
› Author Affiliations
Further Information

Publication History

Received 27 March 2000

Accepted after resubmission 22 January 2001

Publication Date:
12 December 2017 (online)

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Summary

Blood coagulation has been thought to be composed of both intrinsic and extrinsic pathways. Recent evidence strongly supports the critical role of the extrinsic pathway in the initiation of blood coagulation. This investigation established an assay that examines the role of FXI in the thromboplastin-initiated (extrinsic) coagulation based on this new concept. Plasma clotting times were measured at different concentrations of thromboplastin with activated FXII inhibited (FXIIa-inhibited Diluted Thromboplastin Time, FXIIaiDTT). Only at low concentrations of thromboplastin was FXIIaiDTT of FXI-deficient plasma significantly prolonged than that of normal plasma. Depletion of FXI from normal plasma prolonged its FXIIaiDTT and replenishment of FXI shortened it. FXIIaiDTTs of both FVIII-deficient and FIX-deficient plasma were remarkably prolonged, and addition of normal plasma dose-dependently shortened it. Furthermore, earlier α-thrombin inhibition was directly correlated with decreasing FXa generation. The amount of FXa production was: platelet-rich plasma > platelet-poor plasma > FXI-deficient plasma. Therefore, our findings from the FXIIaiDTT assays not only support the critical role of extrinsic pathway in blood coagulation initiation, but also demonstrate the importance of FXI as an amplifier of thrombin generation in thromboplastin-initiated coagulation.

Key words

Blood coagulation - thromboplastin - factor VIII - factor IX - factor XI
 

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