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Nuklearmedizin 1985; 24(03): 146-149
DOI: 10.1055/s-0038-1624294
Short Communications – Kurzmitteilungen
Schattauer GmbH

Investigation of the Parameters Related to the Formation of 99mTc-Pyrophosphate Complexes Using Gel Chromatography Column Scanning

Untersuchung der Parameter zur Bildung von 99mTc-Pyrophosphat-Komplexen mit Gelchromatographie-Säulen-Szintigraphie
Y. F. Shafiq
2   From the Radioisotopes Production Dept., Nuclear Research Centre, Tuwaitha, Baghdad, Iraq
,
M. A. A. Al-Janabi
2   From the Radioisotopes Production Dept., Nuclear Research Centre, Tuwaitha, Baghdad, Iraq
,
H. M. Al-Azzawi
2   From the Radioisotopes Production Dept., Nuclear Research Centre, Tuwaitha, Baghdad, Iraq
,
M. A. A. Abdel-Azeez
2   From the Radioisotopes Production Dept., Nuclear Research Centre, Tuwaitha, Baghdad, Iraq
,
M. H. S. Al-Hissoni
1   The Ibn Al-Nafis Cardiovascular Teaching Hospital, Baghdad, Iraq
,
M. N. Jassim
1   The Ibn Al-Nafis Cardiovascular Teaching Hospital, Baghdad, Iraq
› Author Affiliations
Further Information

Publication History

Received: 14 November 1984

Accepted: 15 March 1985

Publication Date:
20 January 2018 (online)

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Summary

Using gel chromatography two different 99mTc-PPi complexes (complex I and II) were observed under various pH-values. In neutral medium complex II was the main product while in acidic or alkaline media complexes

I and II were formed. Our results related to the organ distribution in mice revealed that complex II

is a bone-seeking agent whereas complex I concentrates in the kidneys.

Zusammenfassung

Bei Anwendung der Gelchromatographie wurden zwei verschiedene 99mTc-PPi-Komplexe (Komplex I und II) bei verschiedenen pH-Werten beobachtet. Im neutralen Bereich war Komplex II das Hauptprodukt, während im sauren oder alkalischen Bereich Komplex I und II gebildet wurde. Anhand der Organverteilung in der Maus konnte gezeigt werden, daß der Komplex II ein knochensuchendes Agens ist, während Komplex I sich in der Niere anreichert.

 

  • References

  • 1 Bowen B. M, Garnett E. S. Analysis of the relationship between 99mTc-Snpolyphosphate and 99mTc-Sn-pyrophosphate. J. nucl. Med. 1974; 15: 652-5.
    PubMedGoogle Scholar
  • 2 Eckelman W. C, Levenson S. M. Radiopharmaceuticals labelled with technetium. Int. J. appl. Radiat. Isot. 1977; 28: 67-82.
    CrossrefPubMedGoogle Scholar
  • 3 Persson R. B.R, Strand S. E. In: Radiopharmaceuticals and Labelled Compounds. Vol. I, 169 IAEA; Vienna: 1973
    Google Scholar
  • 4 Russel C. D, Jerik J E MA, Cash A. G. Technetium pyrophosphate: A mixture? – Preparation of Tc(III) and Tc(IV) pyrophosphates and their comparative biologic properties. J. nucl. Med. 1978; 05: 190-5.
    Google Scholar
  • 5 Schümichen C, Roth K. H, Hoffmann G. Formation and in vivo-distribution of different 99mTc-Sn-pyrophosphate complexes. Nucl. Med. 1975; 14: 323-9.
    Google Scholar
  • 6 Schümichen C, Koch K, Krans A, Kuhlicke G, Weiler K, Wenn A, Hoffmann G. Binding of Technetium-99m to plasma proteins: Influence on the distribution of Tc-99m phosphate agents. J. nucl. Med. 1980; 21: 1080-5.
    PubMedGoogle Scholar
  • 7 Steigman J, Meinken G, Richards P. The reduction of pertechnetate-99 by stannous chloride-II. The stoichimetry of the reaction in aqueous solutions of several phosphorus(V) compounds. Int. J. appl. Radiat. Isot. 1978; 29: 653-60.
    CrossrefGoogle Scholar
  • 8 Subramanian G, McAfee J. G, Bell E. G, Blair R. J, O’Mara R. R, Ralston P. H. 99mTc-labelled polyphosphate as a skeletal imaging agent. Radiology 1972; 102: 701-4.
    CrossrefPubMedGoogle Scholar
  • 9 Van den Brand J. A. G. M, Das H. A, Dekker B. G, de Ligny C. L. Gel chromatography separation and identification of the Tc(Sn)EHDP complexes using the radiotracers 32P, 99mTc and 113Sn. Int. J. appl. Radiat. Isot. 1981; 32: 637-44.
    CrossrefGoogle Scholar