Download PDF
Vet Comp Orthop Traumatol 1999; 12(04): 196-197
DOI: 10.1055/s-0038-1632490
Brief Communication
Schattauer GmbH

A Method of Quantifying Microangiograms

J. W. Wilson
1   From the Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada
,
Mimi Arighi
1   From the Department of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada
› Author Affiliations
Further Information

Publication History

Received 29 July 1998

Accepted 22 March 1999

Publication Date:
08 February 2018 (online)

Also available at
    Permissions and Reprints

Summary

A method is proposed which can quantify the vascularity depicted in microangiograms. Total vascular area depicted in the microangiogram is measured using an image analyser. Vessel diameters and frequency are determined using standard stereological methods. Using the incidence of measured vessel diameters, the measured vascular area, and minimal mathematical manipulation, a distribution curve of possible vascular volumes can be constructed. When combined with sample volume, statistical data relevant to vessel diameters and volumes can be derived. This calculation is not meant as a substitute to stereological methods for quantifying vascularity using thin sections, only as a quantitative adjunct to microangiograms and descriptive microangiography.

A method is proposed which can provide statistical data relevant to vessel diameters and volumes depicted in microangiograms.

Keywords

Microangiogram - vascular volume - vessel diameter - statistical quantification
 

  • REFERENCES

  • 1 Ludders JW, Wilson JW, Ribble GA. Microangiography and correlated histology: a research technique for examining renal microcirculation. Am J Vet Res 1985; 46: 2536-8.
    PubMedSearch in Google Scholar
  • 2 Rhinelander FW, Stewart CL, Wilson JW. Bone vascular supply. In: Skeletal research: an experimental approach (Eds). Simmons DJ, and Kunin AS. New York: Academic Press; 1979: 367-95.
    Search in Google Scholar
  • 3 Rhinelander FW, Wilson JW. Blood supply to developing, mature and healing bone. In: Bone in clinical orthopaedics (Ed.). Sumner- Smith G. Philadelphia: W. B. Saunders Co.,; 1982: 81-158.
    Search in Google Scholar
  • 4 Elias HM. Guide to practical stereology. Karger, Basel: 1983
    Search in Google Scholar