Examination of synovial fluid and serum following intravenous injections of hyaluronan for the treatment of osteoarthritis in dogs

S. O. Canapp; A. R. Cross; M. P. Brown; D. D. Lewis; J. Hernandez; K. A. Merritt; R. Tran-Son-Tay D. Sc

doi:10.1055/s-0038-1632949

Veterinary and Comparative Orthopaedics and Traumatology, Table of Contents

Vet Comp Orthop Traumatol 2005; 18(03): 169-174
DOI: 10.1055/s-0038-1632949

Original Research

Schattauer GmbH

Examination of synovial fluid and serum following intravenous injections of hyaluronan for the treatment of osteoarthritis in dogs

S. O. Canapp

¹Department of Orthopedic Surgery and Sports Medicine, VCA Veterinary Referral Associates, Gaithersburg, Maryland, USA

,

A. R. Cross

²Georgia Veterinary Specialists, Atlanta, Georgia, USA

,

M. P. Brown

³Departments of Large Animal Clinical Sciences

,

D. D. Lewis

⁴Small Animal Clinical Sciences and the Center for Veterinary Sports Medicine, College of Veterinary Medicine

,

J. Hernandez

³Departments of Large Animal Clinical Sciences

,

K. A. Merritt

³Departments of Large Animal Clinical Sciences

,

R. Tran-Son-Tay D. Sc

⁵Department of Mechanical and Aerospace Engineering, College of Engineering, University of Florida, Gainesville, Florida, USA

› Author Affiliations

Abstract

Summary

A randomized, blinded, prospective clinical trial was performed to determine the effects of intravenous (IV) administration of hyaluronan sodium (HA) on serum glycosaminoglycans (GAG) concentrations, synovial fluid (SF) hyaluronan concentrations and viscosity in dogs treated for unilateral rupture of the cranial cruciate ligament. Twenty-two dogs undergoing tibial plateau leveling osteotomy were used in this study. Synovial fluid from both stifles and serum were collected prior to surgery and at 2, 4, and 8 weeks following surgery. Dogs received either 1.0 ml (10 mg) of sodium hyaluronate (treatment group 1; n = 10) or equal volume of 0.9% NaCl (treatment group 2; n = 12), IV immediately, 2 and 4 weeks following surgery. Synovial fluid viscosity was evaluated using a magnetically driven, acoustically tracked, translating-ball rheometer. Synovial fluid HA disaccharide content was measured by fluorophore-assisted carbohydrate electrophoresis. Serum GAG concentrations were measured by alcian blue spectrophotometric assay. Data were analyzed using a Wilcoxon sign rank test (p<0.05). Mean ± SD viscosity (cP) was significantly higher (p=0.011) in SF obtained from the intact stifle (450 ± 604.1) than injured (54.8 ± 60.8) prior to surgery. Mean ± SD HA concentrations (ug/ml) were significantly higher (p=0.02) in synovial fluid obtained from the injured stifles (281.4 ± 145.9) than intact stifles (141.6 ± 132.5). No significant difference was noted within or between treatment groups in SF viscosity, HA concentrations, or serum GAG concentrations at any time following surgery. Stifles with cranial cruciate ligament insufficiency had significant alterations in SF viscosity and HA concentrations.

Keywords

Hyaluronic acid - synovial fluid - osteoarthritis

Full Text

References

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