Circular RNAs in Neuroblastoma

C Danßmann; J Toedling; F Klironomos; A Winkler; F Hertwig; A Eggert; JH Schulte; S Fuchs

doi:10.1055/s-0038-1645000

Klinische Pädiatrie, Table of Contents

Klin Padiatr 2018; 230(03): 169
DOI: 10.1055/s-0038-1645000

Top 3 Solid tumors

Georg Thieme Verlag KG Stuttgart · New York

Circular RNAs in Neuroblastoma

C Danßmann

¹Charité – Universitätsmedizin Berlin

,

J Toedling

¹Charité – Universitätsmedizin Berlin

³German Cancer Consortium (DKTK), Berlin

⁴German Cancer Research Center (DKFZ), Heidelberg; * Authors contributed equally

,

F Klironomos

¹Charité – Universitätsmedizin Berlin

,

A Winkler

¹Charité – Universitätsmedizin Berlin

,

F Hertwig

¹Charité – Universitätsmedizin Berlin

³German Cancer Consortium (DKTK), Berlin

⁴German Cancer Research Center (DKFZ), Heidelberg; * Authors contributed equally

,

A Eggert

¹Charité – Universitätsmedizin Berlin

²Berlin Institute of Health (BIH)

³German Cancer Consortium (DKTK), Berlin

⁴German Cancer Research Center (DKFZ), Heidelberg; * Authors contributed equally

,

JH Schulte

¹Charité – Universitätsmedizin Berlin

²Berlin Institute of Health (BIH)

³German Cancer Consortium (DKTK), Berlin

⁴German Cancer Research Center (DKFZ), Heidelberg; * Authors contributed equally

,

S Fuchs

¹Charité – Universitätsmedizin Berlin

²Berlin Institute of Health (BIH)

³German Cancer Consortium (DKTK), Berlin

⁴German Cancer Research Center (DKFZ), Heidelberg; * Authors contributed equally

› Author Affiliations

Abstract

Full Text

Circular RNAs (circRNA) are a class of non-coding RNA, which originate from back-splicing events during mRNA processing. They are highly abundant in neural tissues and play a role in cancer biology. This project investigates the role of circRNAs in neuroblastoma, the most common extracranial solid tumor of childhood.

We analyzed preliminary RNA sequencing data to identify circRNAs in neuroblastoma. A selection of identified circRNAs was validated in a panel of neuroblastoma cell lines, using qRT-PCR, RNaseR treatment, Sanger sequencing and northern blotting.

We identified 959 circRNAs in the neuroblastoma cell line IMR-5/75, of which many were derived from chromatin-modifying genes. A selected number of these circRNAs was validated.

Overall, we find circRNA expression to be uncorrelated with the host gene mRNA expression, suggesting an independent mechanism regulating circRNAs biogenesis in neuroblastoma.