Intrinsic Activation of Prothrombin Potentiated by Celite and Reduced by Kaolin

E. P Kang; D. C Triantaphyllopoulos

doi:10.1055/s-0038-1647729

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1974; 32(02/03): 600-607
DOI: 10.1055/s-0038-1647729

Original Article

Schattauer GmbH

Intrinsic Activation of Prothrombin Potentiated by Celite and Reduced by Kaolin^[*]

E. P Kang

¹American National Red Cross Blood Research Laboratory, Bethesda, Maryland 20014 and Department of Physiology and Biophysics Georgetown University School of Medicine, Washington, D. C. 20007

,

D. C Triantaphyllopoulos

¹American National Red Cross Blood Research Laboratory, Bethesda, Maryland 20014 and Department of Physiology and Biophysics Georgetown University School of Medicine, Washington, D. C. 20007

› Author Affiliations

Abstract

Summary

Preparations of human prothrombin complex were absorbed with celite or kaolin and the 10% sodium chloride eluate of both absorbents was tested for its effectiveness to correct the prolonged partial thromboplastin time of celite-exhausted plasma. Both types of eluate were effective, indicating the presence of contact factors in the prothrombin preparations. Activation of the absorbed prothrombin complexes via the intrinsic pathway showed that while the kaolin absorbed preparations generated less thrombin than the parent solutions, celite absorbed complexes generated more thrombin than before they were absorbed. Both types of absorbed preparations generated the same amount of thrombin as before absorption when they were activated via the extrinsic pathway. When the absorption of the contact factors was performed by the addition of celite in the plasma before the purification of the prothrombin complex, the prothrombin preparations obtained generated less thrombin via the intrinsic pathway than the preparations which were obtained from plasma which had not been treated with celite.

Full Text

References

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Intrinsic Activation of Prothrombin Potentiated by Celite and Reduced by Kaolin[*]

Intrinsic Activation of Prothrombin Potentiated by Celite and Reduced by Kaolin^[*]