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Thromb Haemost 1973; 29(02): 450-460
DOI: 10.1055/s-0038-1647785
Original Article
Schattauer GmbH

Lipid Infusions in Man

Ultrastructural Studies on Blood Platelet Uptake of Fat Particles
T Hovig
1   Institute of Pathology, Electron Microscopic Laboratory (Chief, Spesiallege T. Hovig) and Section of Haematology (Chief, Professor P. F. Hjort), Medical Department A (Chief, Professor E. Gjone), Rikshospitalet, Oslo 1, Norway
,
K. A Grøttum
1   Institute of Pathology, Electron Microscopic Laboratory (Chief, Spesiallege T. Hovig) and Section of Haematology (Chief, Professor P. F. Hjort), Medical Department A (Chief, Professor E. Gjone), Rikshospitalet, Oslo 1, Norway
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Publication History

Received for publication 02 October 1972

Publication Date:
30 June 2018 (online)

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Summary

The soybean lipid emulsion “Intralipid” was infused in 10 volunteer individuals, and platelets, obtained up to 22 hours after the infusions, were studied by transmission and scanning electron microscopy. Using negative staining technique, platelet coating with lipid particles could be demonstrated. Platelet engulf ment of the lipid particles was found in ultrathin sections. The lipid particles appeared to enter the platelets via invaginations of the surface membranes and were located in vacuoles and in’ ‘humps”. The platelet shape and internal structure was strikingly well preserved and no evidence of platelet aggregation was demonstrated. Small platelet cytoplasmic fragments containing lipid were observed, and it is suggested that these may be pinched off from the platelets. Both with negative staining and in scanning electron micrographs occasional clumps of lipid particles were observed, and the possibility can not be excluded that such clumps may interfere with microcirculatory flow.

 

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