Platelet Preservation. III. The Influence of Dimethylsulfoxide and Cooling Conditions on Serotonin Uptake Velocity and Response to Hypotonic Stress of Human Platelets

J Odink; R Sprokholt

doi:10.1055/s-0038-1648024

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1976; 36(01): 192-199
DOI: 10.1055/s-0038-1648024

Original Article

Schattauer GmbH

Platelet Preservation. III. The Influence of Dimethylsulfoxide and Cooling Conditions on Serotonin Uptake Velocity and Response to Hypotonic Stress of Human Platelets

J Odink

¹Central Laboratory of the Netherlands’ Red Cross Blood Transfusion Service, Plesmanlaan 125, P.O.B. 9190, Amsterdam, The Netherlands

,

R Sprokholt

¹Central Laboratory of the Netherlands’ Red Cross Blood Transfusion Service, Plesmanlaan 125, P.O.B. 9190, Amsterdam, The Netherlands

› Author Affiliations

Abstract

Summary

1. Serotonin uptake velocity of human platelets was found to be reversibly inhibited by the presence of dimethylsulfoxide (DMSO).

2. Incubation of platelet concentrates in the presence of DMSO for 90 minutes at room temperature decreased serotonin uptake velocity and response to hypotonic stress.

3. The serotonin uptake velocity was not substantially influenced by the temperature of incubation with DMSO.

4. The best recovery of serotonin uptake velocity after cryopreservation in the presence of DMSO was observed when DMSO was added quickly before cooling and removed quickly after thawing.

5. No significant differences were observed in serotonin uptake velocity and response to hypotonic stress after cryopreservation in the presence of DMSO when cooling rates were varied from 2 to 10° C/min.

Full Text

References

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