Preparation of Modified Bovine Factor VIII with Enhanced Biological Activity Using Insoluble-trypsin Columns

Christine N. Vogel; Henry E. Parfitt Jr.; Henry S. Kingdon; Roger L. Lundblad

doi:10.1055/s-0038-1649069

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1973; 30(01): 229-234
DOI: 10.1055/s-0038-1649069

Original Article

Schattauer GmbH

Preparation of Modified Bovine Factor VIII with Enhanced Biological Activity Using Insoluble-trypsin Columns

Authors

Christine N. Vogel

¹Dental Research Center and the Departments of Pathology, Biochemistry, and Medicine, University of North Carolina, Chapel Hill, North Carolina 27514 (USA)
Henry E. Parfitt Jr.

¹Dental Research Center and the Departments of Pathology, Biochemistry, and Medicine, University of North Carolina, Chapel Hill, North Carolina 27514 (USA)
Henry S. Kingdon

¹Dental Research Center and the Departments of Pathology, Biochemistry, and Medicine, University of North Carolina, Chapel Hill, North Carolina 27514 (USA)
Roger L. Lundblad

¹Dental Research Center and the Departments of Pathology, Biochemistry, and Medicine, University of North Carolina, Chapel Hill, North Carolina 27514 (USA)

Abstract

Summary

Crude bovine Factor VIII preparations have been modified by passage over a column of trypsin-agarose. Factor VIII prepared without an early adsorption step utilizing either barium sulfate or aluminum hydroxide gel generated significant Factor Xa activity by passage over such a column and was unsuitable for further characterization. Factor VIII prepared using adsorption with barium sulfate contained no Factor Xa activity after passage over trypsin-agarose. This trypsin-modified material showed a decreased molecular size as judged by behavior on gel filtration and an increased rate of reaction with bovine Factor IXa.

Full Text

References

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