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Thromb Haemost 1973; 30(01): 138-147
DOI: 10.1055/s-0038-1649110
Original Article
Schattauer GmbH

The Filter Loop Technique as a Method of Measuring Platelet Aggregation in the Flowing Blood of the Rat; the Inhibitory Activity of 5-oxo-l-cyclopentene-l-heptanoic Acid (AY-16,804) on Platelet Aggregation[*]

Christopher R. Muirhead Ph.D.
1   Department of Pharmacology, Ay erst Research Laboratories, P. O. Box 6115, Montreal 101, Quebec, Canada
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Further Information

Publication History

Received for publication 06 February 1973

Accepted for publication 22 March 1973

Publication Date:
30 June 2018 (online)

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Summary

The filter loop technique which measures platelet aggregation in vivo in the flowing-blood of the rat was compared to the optical density technique of Born which is carried out in vitro with platelet rich plasma. Using these two experimental models the effect on platelet aggregation of three known inhibitors sulfinpyrazone, dipyridamole and prostaglandin E1, and a novel compound 5-oxo-l-cyclopentene-l-heptanoic acid (AY-16, 804) was determined.

The effects on platelet aggregation of the known inhibitors were consistent with information in the literature. Prostaglandin E1 was the most potent inhibitor in both techniques; sulfinpyrazone inhibited aggregation in both models but was less potent than prostaglandin E1. AY-16, 804 exhibited activity in vitro and in vivo similar to that of sulfinpyrazone. Dipyridamole did not inhibit platelet aggregation in vivo and did not inhibit aggregation in vitro in concentrations at which it remained soluble.

The filter loop technique is a suitable model for measuring platelet aggregation in the flowing blood of the rat. It is a relatively simple method of determining aggregation and easily adapted to other species.

* Presented in part at the 56th Annual Meeting of the Federation of American Societies for Experimental Biology, Atlantic City, N. J., April 9-14, 1972.


 

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