Reaction Characteristics of Antiprothrombin Antibody on the Activation of Prothrombin (Sheep)^[*]

Summary

1. Prothrombin was purified by a series of steps and subsequently on DEAE-Cellu- lose chromatography and specific antibody to pure prothrombin was prepared.

2. The effect of Anti-prothrombin antibody was tested on the activation of prothrombin. The generated thrombin was assayed for its clotting function as well as for esterase activity on TAMe (Tosyl-Arginine-Methyl ester) as substrate.

3. Anti-prothrombin antibody inhibits the clotting function of generated thrombin completely but its esterase function is only partially affected.

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