Thrombosis and Haemostasis, Inhaltsverzeichnis Thromb Haemost 1972; 27(02): 272-277DOI: 10.1055/s-0038-1649366 Originalarbeiten — Original Articles — Travaux Originaux Schattauer GmbH Glutaraldehyde Fixation of Human Blood Platelets J. J Sixma * Division of Haemostasis and Thrombosis, State University, Utrecht, The Netherlands , W. H Linssen ** Department of Internal Medicine and Center for Medical Electron Microscopy, State University, Utrecht, The Netherlands , H. J Geuze ** Department of Internal Medicine and Center for Medical Electron Microscopy, State University, Utrecht, The Netherlands › Institutsangaben Artikel empfehlen Abstract Artikel einzeln kaufen Summary Hypertonic glutaraldehyde solutions produce dilatation of the surface connected system and shrinking of the cytoplasma. Hypertonic solutions produce swelling of the platelets. Good results are obtained with 1% glutaraldehyde M 0.07 M phosphate buffer (275 mosmol) 4 parts to 1 part platelet rich plasma. High glutaraldehyde concentrations do not affect the platelet ultrastructure. Volltext Referenzen References 1 Avrameas S, Ternynck T. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immnno-absorbents. Immunochem 06: 53-66. 1969; 2 Behnke O. Electron microscopic observations on the membrane system of the rat blood platelets. Anat. Rec 158: 121-137. 1967; 3 Behnke O. Effects of some chemicals on the blood platelet microtubules, platelet shape and some platelet function in vitro. Scand. J. Haemat 07: 123-140. 1970; 4 Maunsbach A. B. The influence of different fixatives and fixation methods on the ultrastructure of the rat kidney proximal tubule cell II. J. Ultrastruct. Res 15: 283-309. 1966; 5 Pease D. C. Histological techniques for electron microscopy. 2nd edit. Academic Press; New York-London: 1964 6 Reynolds E. S. The use of the lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol 17: 208-212. 1963; 7 Sabatini D. D, Bensch K, Barnett R. The preservation of cellular ultrastructure and enzymatic activity of aldehyde fixation. J. Cell Biol 17: 19 1963; 8 Silver M. D, Gardner H. A. Variations in rabbit platelet morphology induced by different aldehyde prefixatives. Pathology 02: 199-207. 1970; 9 Sixma J. J, Molenaar I. Microtubules and microfibrils in human platelets. Thrombos. Diathes. haemorrh. (Stuttg) 16: 153-161. 1966; 10 Watson M. L. Staining of tissue sections for electron microscopy with heavy metals. J. bio- phys. biochem. Cytol 04: 475-479. 1958; 11 Zetterqvist H. The ultrastructural organisation of the columnar absorbing cells of the mouse jejunum. Thesis; Karolinska Institutet: 1956
