Rapid Detection of Fibrinopeptides by Bidimensional Paper Electrophoresis

K Stocker; P. W Straub; B Eckert

doi:10.1055/s-0038-1654231

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1970; 24(01/02): 248-255
DOI: 10.1055/s-0038-1654231

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

Rapid Detection of Fibrinopeptides by Bidimensional Paper Electrophoresis

K Stocker

¹Laboratories of Pentapharm AG, Basle, and the Department of Medicine, Kantonsspital, University of Zurich, Switzerland

,

P. W Straub with the technical assistance of

¹Laboratories of Pentapharm AG, Basle, and the Department of Medicine, Kantonsspital, University of Zurich, Switzerland

,

B Eckert

¹Laboratories of Pentapharm AG, Basle, and the Department of Medicine, Kantonsspital, University of Zurich, Switzerland

› Author Affiliations

Abstract

Summary

A simplified method for the rapid identification of the fibrinopeptides in small amounts of supernatants of fibrin clots is described.

Using this method, we confirmed the earlier observation that Reptilase-R, a thrombin-like enzyme from the venom of Bothrops atrox, exclusively liberates fibrinopeptides A and AP. It was further demonstrated that fibrinopeptide B can be rapidly isolated from small amounts of fibrinogen or even of plasma by thrombin action on washed Reptilase clots.

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References

References
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