Density Gradient Centrifugation and Electron Microscopic Characterization of Subcellular Fractions from Human Blood Platelets

A Siegel; P. H Burri; E. R Weibel; M Bettex-Galland; E. F Lüscher

doi:10.1055/s-0038-1654300

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1971; 25(02): 252-267
DOI: 10.1055/s-0038-1654300

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

Density Gradient Centrifugation and Electron Microscopic Characterization of Subcellular Fractions from Human Blood Platelets

A Siegel

¹Theodor Kocher Institute and Department of Anatomy, University of Berne

,

P. H Burri

¹Theodor Kocher Institute and Department of Anatomy, University of Berne

,

E. R Weibel

¹Theodor Kocher Institute and Department of Anatomy, University of Berne

,

M Bettex-Galland

¹Theodor Kocher Institute and Department of Anatomy, University of Berne

,

E. F Lüscher

¹Theodor Kocher Institute and Department of Anatomy, University of Berne

› Author Affiliations

Abstract

Summary

Homogenized human blood platelets have been fractionated by centrifugation in Ficoll and sucrose density gradients. The different fractions were examined by electron microscopy.

Although Ficoll allows for the separation of very distinct zones, its ability to form complexes with cellular components made sucrose the preferable gradient. Sucrose, in spite of its unfavorable osmotic effect, allows for an acceptable fractionation of platelet components.

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References

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