Metabolic Formation of a Prothrombin Derivative^[*]

Summary

The administration of coumadin to dogs on consecutive days was followed by the simultaneous decrease in prothrombin and autoprothrombin III (F-X) concentration. The decline lasted 4 days. Prethrombin concentration increased. Purified prothrombin complex was infused, while the anticoagulant was still blocking synthesis. This restored the original concentration of prothrombin and autoprothrombin III. Each one of these disappeared in one day while prethrombin concentration temporarily increased and then also declined. Infused prethrombin was cleared in less than 1 day. Human prothrombin complex was also infused intravenously with the same results. Similar results were found with the use of rabbits. A derivative of prothrombin, which is not detected with the two-stage analytical reagents, is normally in plasma and is derived from the prothrombin in the plasma which is responsive to the two-stage analytical reagents. After inhibiting the synthesis of prothrombin and autoprothrombin III, these disappeared far more slowly from plasma than the purified prothrombin and autoprothrombin III which was infused.

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